Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Metabolomics reveals metabolic changes in male reproductive cells exposed to thirdhand smoke

Journal Article · · Scientific Reports
DOI:https://doi.org/10.1038/srep15512· OSTI ID:1256060
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [3];  [3];  [1]
  1. Nanjing Medical Univ., Nanjing (China)
  2. Univ. of California, San Francisco, CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division
+ Show Author Affiliations
Thirdhand smoke (THS) is a new term for the toxins in cigarette smoke that linger in the environment long after the cigarettes are extinguished. The effects of THS exposure on male reproduction have not yet been studied. In this study, metabolic changes in male germ cell lines (GC-2 and TM-4) were analyzed after THS treatment for 24 h. THS-loaded chromatography paper samples were generated in a laboratory chamber system and extracted in DMEM. At a paper: DMEM ratio of 50 μg/ml, cell viability in both cell lines was normal, as measured by the MTT assay and markers of cytotoxicity, cell cycle, apoptosis and ROS production were normal as measured by quantitative immunofluorescence. Metabolomic analysis was performed on methanol extracts of GC-2 and TM-4 cells. Furthermore, glutathione metabolism in GC-2 cells, and nucleic acid and ammonia metabolism in TM-4 cells, was changed significantly by THS treatment. RT-PCR analyses of mRNA for enzyme genes Gss and Ggt in GC-2 cells, and TK, SMS and Glna in TM-4 cells reinforced these findings, showing changes in the levels of enzymes involved in the relevant pathways. In conclusion, exposure to THS at very low concentrations caused distinct metabolic changes in two different types of male reproductive cell lines.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE; USDOE Office of Science (SC)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1256060
Alternate ID(s):
OSTI ID: 1378601
Journal Information:
Scientific Reports, Journal Name: Scientific Reports Vol. 5; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

References (33)

Glutathione metabolism and its role in hepatotoxicity journal December 1991
Genotoxicity of tobacco smoke and tobacco smoke condensate: a review journal November 2004
Genotoxicity of environmental tobacco smoke: a review journal November 2004
Disorders of purine and pyrimidine metabolism journal September 2005
Second-hand smoke and human lung cancer journal July 2008
Cardiovascular Effects of Secondhand Smoke: Nearly as Large as Smoking journal May 2005
Thirdhand Tobacco Smoke: Emerging Evidence and Arguments for a Multidisciplinary Research Agenda journal September 2011
Association between parental smoking behavior and children's respiratory morbidity: 5-year study in an urban city of South Korea journal October 2011
Glutathione metabolism and its role in hepatotoxicity journal December 1991
Ammonia toxicity in fish journal September 2002
Second-hand smoke and human lung cancer journal July 2008
Lipid peroxidation and antioxidant responses in zebrafish brain induced by Aphanizomenon flos-aquae DC-1 aphantoxins journal November 2013
Involvement of activating ERK1/2 through G protein coupled receptor 30 and estrogen receptor α/β in low doses of bisphenol A promoting growth of Sertoli TM4 cells journal April 2014
Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression journal February 2009
Metabolomic profiles delineate the potential role of glycine in gold nanorod-induced disruption of mitochondria and blood–testis barrier factors in TM-4 cells journal January 2014
Formation of carcinogens indoors by surface-mediated reactions of nicotine with nitrous acid, leading to potential thirdhand smoke hazards journal February 2010
A Combined Proteomics and Metabolomics Profiling of Gastric Cardia Cancer Reveals Characteristic Dysregulations in Glucose Metabolism journal August 2010
Residual tobacco smoke pollution in used cars for sale: Air, dust, and surfaces journal September 2008
Human urinary carcinogen metabolites: biomarkers for investigating tobacco and cancer journal June 2002
Doses of Nicotine and Lung Carcinogens Delivered to Cigarette Smokers journal January 2000
Thirdhand smoke causes DNA damage in human cells journal March 2013
Interlaboratory comparability of serum cotinine measurements at smoker and nonsmoker concentration levels: A round-robin study journal November 2009
When smokers move out and non-smokers move in: residential thirdhand smoke pollution and exposure journal October 2010
Thirdhand cigarette smoke in an experimental chamber: evidence of surface deposition of nicotine, nitrosamines and polycyclic aromatic hydrocarbons and de novo formation of NNK journal May 2013
Relationship of Human Toenail Nicotine, Cotinine, and 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanol to Levels of These Biomarkers in Plasma and Urine journal July 2007
Concentrations of the Carcinogen 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone in Sidestream Cigarette Smoke Increase after Release into Indoor Air: Results from Unpublished Tobacco Industry Research journal August 2007
Cardiovascular Effects of Secondhand Smoke: Nearly as Large as Smoking journal May 2005
Effects of Maternal Smoking during Pregnancy and Environmental Tobacco Smoke on Asthma and Wheezing in Children journal February 2001
Oxidative Stress Produced by Marijuana Smoke: An Adverse Effect Enhanced by Cannabinoids journal June 1999
Inhibition of salivary enzymes by cigarette smoke and the protective role of glutathione journal January 2002
Bisphenol A Alters n-6 Fatty Acid Composition and Decreases Antioxidant Enzyme Levels in Rat Testes: A LC-QTOF-Based Metabolomics Study journal September 2012
Cigarette Smoke Toxins Deposited on Surfaces: Implications for Human Health journal January 2014
Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells journal September 2013

Cited By (5)

Experimental Acute Exposure to Thirdhand Smoke and Changes in the Human Nasal Epithelial Transcriptome journal June 2019
Short-term early exposure to thirdhand cigarette smoke increases lung cancer incidence in mice journal February 2018
Early exposure to thirdhand cigarette smoke affects body mass and the development of immunity in mice journal February 2017
Thirdhand Smoke at Philip Morris journal July 2018
Thirdhand smoke: when the danger is more than you can see or smell journal November 2016

Similar Records

Adverse Health Effects of Thirdhand Smoke: From Cell to Animal Models
Journal Article · Thu Apr 27 20:00:00 EDT 2017 · International Journal of Molecular Sciences (Online) · OSTI ID:1408431
Early exposure to thirdhand cigarette smoke affects body mass and the development of immunity in mice
Journal Article · Thu Feb 02 19:00:00 EST 2017 · Scientific Reports · OSTI ID:1379722

Related Subjects

59 BASIC BIOLOGICAL SCIENCES