OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice

Li, Fengcheng; Xie, Guosheng; Huang, Jiangfeng; Zhang, Ran; Li, Yu; Zhang, Miaomiao; Wang, Yanting; Li, Ao; Li, Xukai; Xia, Tao; Qu, Chengcheng; Hu, Fan; Ragauskas, Arthur J.; Peng, Liangcai

doi:10.1111/pbi.12700

Title: OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice

Journal Article · Wed Mar 15 00:00:00 EDT 2017 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12700· OSTI ID:1376598

Li, Fengcheng ^[1]; Xie, Guosheng ^[1]; Huang, Jiangfeng ^[1]; Zhang, Ran ^[1]; Li, Yu ^[1]; Zhang, Miaomiao ^[1]; Wang, Yanting ^[1]; Li, Ao ^[1]; Li, Xukai ^[1]; Xia, Tao ^[1]; Qu, Chengcheng ^[1]; Hu, Fan ^[2];

^[2]; Peng, Liangcai ^[1]

Huazhong Agricultural Univ. Wuhan (China)
Univ. of Tennessee, Knoxville, TN (United States)

Genetic modification of plant cell walls has been posed to reduce lignocellulose recalcitrance for enhancing biomass saccharification. Several dozen CESA mutants have been reported since cellulose synthase (CESA) gene was first identified, but almost all mutants exhibit the defective phenotypes in plant growth and development. Here, the rice (Oryza sativa) Osfc16 mutant with substitutions (W481C, P482S) at P-CR conserved site in CESA9 shows a slightly affected plant growth and higher biomass yield by 25%–41% compared with wild type (Nipponbare, a japonica variety). Chemical and ultrastructural analyses indicate that Osfc16 has a significantly reduced cellulose crystallinity (CrI) and thinner secondary cell walls compared with wild type. CESA co-IP detection, together with implementations of a proteasome inhibitor (MG132) and two distinct cellulose inhibitors (Calcofluor, CGA), shows that CESA9 mutation could affect integrity of CESA4/7/9 complexes, which may lead to rapid CESA proteasome degradation for low-DP cellulose biosynthesis. These may reduce cellulose CrI, which improves plant lodging resistance, a major and integrated agronomic trait on plant growth and grain production, and enhances biomass enzymatic saccharification by up to 2.3-fold and ethanol productivity by 34%–42%. Our study has for the first time reported a direct modification for the low-DP cellulose production that has broad applications in biomass industries.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1376598

Journal Information:: Plant Biotechnology Journal, Vol. 15, Issue 9; ISSN 1467-7644

Publisher:: Society for Experimental Biology; Association of Applied BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 85 works

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Title: OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice

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