A finalized determinant for complete lignocellulose enzymatic saccharification potential to maximize bioethanol production in bioenergy Miscanthus

Alam, Aftab; Zhang, Ran; Liu, Peng; Huang, Jiangfeng; Wang, Yanting; Hu, Zhen; Madadi, Meysam; Sun, Dan; Hu, Ruofei; Ragauskas, Arthur J.; Tu, Yuanyuan; Peng, Liangcai

doi:10.1186/s13068-019-1437-4

A finalized determinant for complete lignocellulose enzymatic saccharification potential to maximize bioethanol production in bioenergy Miscanthus

Journal Article · Sat Apr 27 00:00:00 EDT 2019 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-019-1437-4· OSTI ID:1606669

Alam, Aftab ^[1]; Zhang, Ran ^[1]; Liu, Peng ^[1]; Huang, Jiangfeng ^[1]; Wang, Yanting ^[1]; Hu, Zhen ^[1]; Madadi, Meysam ^[1]; Sun, Dan ^[2]; Hu, Ruofei ^[3]; ^[4]; Tu, Yuanyuan ^[1]; ^[1]

Huazhong Agricultural Univ., Wuhan (China). College of Plant Science and Technology and Biomass and Bioenergy Research Centre
Huazhong Agricultural Univ., Wuhan (China). College of Plant Science; Hubei Univ. of Technology, Wuhan (China). School of Materials and Chemical Engineering
Hubei Univ. of Arts and Science, Xiangyang (China). College of Food Science and Technology
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering

Miscanthus is a leading bioenergy crop with enormous lignocellulose production potential for biofuels and chemicals. However, lignocellulose recalcitrance leads to biomass process difficulty for an efficient bioethanol production. Hence, it becomes essential to identify the integrative impact of lignocellulose recalcitrant factors on cellulose accessibility for biomass enzymatic hydrolysis. In this study, we analyzed four typical pairs of Miscanthus accessions that showed distinct cell wall compositions and sorted out three major factors that affected biomass saccharification for maximum bioethanol production. Among the three optimal (i.e., liquid hot water, H₂SO₄ and NaOH) pretreatments performed, mild alkali pretreatment (4% NaOH at 50 °C) led to almost complete biomass saccharification when 1% Tween-80 was co-supplied into enzymatic hydrolysis in the desirable Miscanthus accessions. Consequently, the highest bioethanol yields were obtained at 19% (% dry matter) from yeast fermentation, with much higher sugar–ethanol conversion rates by 94–98%, compared to the other Miscanthus species subjected to stronger pretreatments as reported in previous studies. By comparison, three optimized pretreatments distinctively extracted wall polymers and specifically altered polymer features and inter-linkage styles, but the alkali pretreatment caused much increased biomass porosity than that of the other pretreatments. Based on integrative analyses, excellent equations were generated to precisely estimate hexoses and ethanol yields under various pretreatments and a hypothetical model was proposed to outline an integrative impact on biomass saccharification and bioethanol production subjective to a predominate factor (CR stain) of biomass porosity and four additional minor factors (DY stain, cellulose DP, hemicellulose X/A, lignin G-monomer). Using four pairs of Miscanthus samples with distinct cell wall composition and varied biomass saccharification, this study has determined three main factors of lignocellulose recalcitrance that could be significantly reduced for much-increased biomass porosity upon optimal pretreatments. It has also established a novel standard that should be applicable to judge any types of biomass process technology for high biofuel production in distinct lignocellulose substrates. Hence, this study provides a potential strategy for precise genetic modification of lignocellulose in all bioenergy crops.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1606669

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 12; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Additional file 11 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053143.v1	image	January 2021
Additional file 12 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053146.v1	image	January 2021
Additional file 13 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053149	image	January 2021
Additional file 14 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053152	image	January 2021
Additional file 15 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053155.v1	image	January 2021
Additional file 16 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053158.v1	image	January 2021
Additional file 1 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053161.v1	dataset	January 2021
Additional file 3 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053167	image	January 2021
Additional file 4 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053170	image	January 2021
Additional file 5 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053173.v1	image	January 2021
Additional file 6 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053176	image	January 2021
Additional file 7 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053179	image	January 2021
Additional file 8 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053182.v1	image	January 2021
Additional file 9 of A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice Ye, Yafeng; Wang, Shuoxun; Wu, Kun figshare https://doi.org/10.6084/m9.figshare.14053185.v1	image	January 2021
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MOESM1 of A finalized determinant for complete lignocellulose enzymatic saccharification potential to maximize bioethanol production in bioenergy Miscanthus Alam, Aftab; Zhang, Ran; Liu, Peng https://doi.org/10.6084/m9.figshare.8051141 The current record is supplemented by this audiovisual	audiovisual	January 2019
MOESM1 of A finalized determinant for complete lignocellulose enzymatic saccharification potential to maximize bioethanol production in bioenergy Miscanthus Alam, Aftab; Zhang, Ran; Liu, Peng https://doi.org/10.6084/m9.figshare.8051141.v1 The current record is supplemented by this audiovisual	audiovisual	January 2019
A finalized determinant for complete lignocellulose enzymatic saccharification potential to maximize bioethanol production in bioenergy Miscanthus Alam, Aftab; Zhang, Ran; Liu, Peng https://doi.org/10.6084/m9.figshare.c.4486433 The current record is supplemented by this collection	collection	January 2019
A finalized determinant for complete lignocellulose enzymatic saccharification potential to maximize bioethanol production in bioenergy Miscanthus Alam, Aftab; Zhang, Ran; Liu, Peng https://doi.org/10.6084/m9.figshare.c.4486433.v1 The current record is supplemented by this collection	collection	January 2019

A finalized determinant for complete lignocellulose enzymatic saccharification potential to maximize bioethanol production in bioenergy Miscanthus

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