Absolute Intra-Molecular Distance Measurements with Ångström-Resolution using Anomalous Small-Angle X-ray Scattering

Zettl, Thomas; Mathew, Rebecca S.; Seifert, Sönke; Doniach, Sebastian; Harbury, Pehr A.B.; Lipfert, Jan

doi:10.1021/acs.nanolett.6b01160

Absolute Intra-Molecular Distance Measurements with Ångström-Resolution using Anomalous Small-Angle X-ray Scattering

Journal Article · Tue May 31 00:00:00 EDT 2016 · Nano Letters

DOI:https://doi.org/10.1021/acs.nanolett.6b01160· OSTI ID:1373387

Zettl, Thomas ^[1]; Mathew, Rebecca S. ^[2]; Seifert, Sönke ^[3]; Doniach, Sebastian ^[4]; Harbury, Pehr A.B. ^[5]; Lipfert, Jan ^[1]

Ludwig Maximilian Univ., Munich (Germany). Nanosystems Initiative Munich, and Center for Nanoscience, Dept. of Physics
Harvard Univ., Boston, MA (United States). Harvard Medical School, Dept. of Cell Biology
Argonne National Lab. (ANL), Argonne, IL (United States). X-ray Science Division
Stanford Univ., CA (United States). Dept. of Applied Physics and Physics
Stanford Univ., CA (United States). Dept. of Biochemistry

Accurate determination of molecular distances is fundamental to understanding the structure, dynamics, and conformational ensembles of biological macromolecules. Here we present a method to determine the full,distance,distribution between small (~7 Å) gold labels attached to macromolecules with very high-precision(≤1 Å) and on an absolute distance scale. Our method uses anomalous small-angle X-ray scattering close to a gold absorption edge to separate the gold-gold interference pattern from other scattering contributions. Results for 10-30 bp DNA constructs achieve excellent signal-to-noise and are in good agreement with previous results obtained by single-energy,SAXS measurements without requiring the preparation and measurement of single labeled and unlabeled samples. Finally, the use of small gold labels in combination with ASAXS read out provides an attractive approach to determining molecular distance distributions that will be applicable to a broad range of macromolecular systems.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1373387

Journal Information:: Nano Letters, Journal Name: Nano Letters Journal Issue: 9 Vol. 16; ISSN 1530-6984

Country of Publication:: United States

Language:: English

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High-throughput AFM analysis reveals unwrapping pathways of H3 and CENP-A nucleosomes Konrad, Sebastian F.; Vanderlinden, Willem; Frederickx, Wout bioRxiv https://doi.org/10.1101/2020.04.09.034090	journal	April 2020
Gold nanocrystal labels provide a sequence–to–3D structure map in SAXS reconstructions Zettl, Thomas; Mathew, Rebecca S.; Shi, Xuesong Science Advances, Vol. 4, Issue 5 https://doi.org/10.1126/sciadv.aar4418	journal	May 2018
Biological small-angle neutron scattering: recent results and development Mahieu, Emilie; Gabel, Frank Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 8 https://doi.org/10.1107/s2059798318005016	journal	July 2018

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47 OTHER INSTRUMENTATION
DNA
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SAXS
anomalous small-angle X-ray scattering
gold nanocrystals
molecular ruler

Absolute Intra-Molecular Distance Measurements with Ångström-Resolution using Anomalous Small-Angle X-ray Scattering

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