Metal-assisted polyatomic SIMS and laser desorption/ionization for enhanced small molecule imaging of bacterial biofilms

Dunham, Sage J.  B.; Comi, Troy J.; Ko, Kyungwon; Li, Bin; Baig, Nameera F.; Morales-Soto, Nydia; Shrout, Joshua D.; Bohn, Paul W.; Sweedler, Jonathan V.

doi:10.1116/1.4942884

Title: Metal-assisted polyatomic SIMS and laser desorption/ionization for enhanced small molecule imaging of bacterial biofilms

Journal Article · Fri Mar 04 00:00:00 EST 2016 · Biointerphases

DOI:https://doi.org/10.1116/1.4942884· OSTI ID:1371711

Dunham, Sage J. B. ^[1]; Comi, Troy J. ^[1]; Ko, Kyungwon ^[1]; Li, Bin ^[1]; Baig, Nameera F. ^[2]; Morales-Soto, Nydia ^[3]; Shrout, Joshua D. ^[3]; Bohn, Paul W. ^[2]; Sweedler, Jonathan V. ^[4]

Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Chemistry and Beckman Inst. for Advanced Science and Technology
Univ. of Notre Dame, IN (United States). Dept. of Chemistry and Biochemistry, Dept. of Chemical and Biomolecular Engineering
Univ. of Notre Dame, IN (United States). Dept. of Civil and Environmental Engineering and Earth Sciences, Dept. of Biological Sciences, Eck Inst. for Global Health
Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Chemistry, Beckman Inst. for Advanced Science and Technology

Mass spectrometry imaging (MSI) has become an important analytical tool for many sectors of science and medicine. As the application of MSI expands into new areas of inquiry, existing methodologies must be adapted and improved to meet emerging challenges. In particular salient is the need for small molecule imaging methods that are compatible with complex multicomponent systems, a challenge that is amplified by the effects of analyte migration and matrix interference. Furthermore, with a focus on microbial biofilms from the opportunistic pathogen Pseudomonas aeruginosa, the relative advantages of two established microprobe-based MSI techniques—polyatomic secondary ion mass spectrometry (SIMS) and laser desorption/ionization—are compared, with emphasis on exploring the effect of surface metallization on small molecule imaging. A combination of qualitative image comparison and multivariate statistical analysis demonstrates that sputtering microbial biofilms with a 2.5 nm layer of gold selectively enhances C₆₀-SIMS ionization for several molecular classes including rhamnolipids and 2-alkyl-quinolones. Metallization also leads to the reduction of in-source fragmentation and subsequent ionization of media-specific background polymers, which improves spectral purity and image quality. Our findings show that the influence of metallization upon ionization is strongly dependent on both the surface architecture and the analyte class, and further demonstrate that metal-assisted C₆₀-SIMS is a viable method for small molecule imaging of intact molecular ions in complex biological systems. VC 2016 American Vacuum Society.

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Research Organization:: University of Notre Dame, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0006642; DBI-9871103; R01AI113219; T32 GM70421; DE SC-0006642

OSTI ID:: 1371711

Alternate ID(s):: OSTI ID: 1421229

Journal Information:: Biointerphases, Vol. 11, Issue 2; ISSN 1934-8630

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 19 works

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