In Situ Correlated Molecular Imaging of Chemically Communicating Microbial Communities

Bohn, Paul W.; Shrout, J. D.; Sweedler, J. V.; Farrand, S.

doi:10.2172/1235677

In Situ Correlated Molecular Imaging of Chemically Communicating Microbial Communities

Technical Report · Mon Jan 25 04:00:00 EST 2016

DOI:https://doi.org/10.2172/1235677· OSTI ID:1235677

Bohn, Paul W. ^[1]; Shrout, J. D. ^[2]; Sweedler, J. V. ^[3]; Farrand, S. ^[3]

Univ. of Notre Dame, IN (United States); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Univ. of Notre Dame, IN (United States)
Univ. of Illinois, Urbana-Champaign, IL (United States)

This document constitutes the final technical report for DE-SC0006642, In Situ Correlated Molecular Imaging of Chemically Communicating Microbial Communities, a project carried out collaboratively by investigators at Notre Dame and UIUC. The work carried out under DOE support in this project produced advances in two areas: development of new highly sophisticated correlated imaging approaches and the application of these new tools to the growth and differentiation of microbial communities under a variety of environmental conditions. A significant effort involved the creation of technical enhancements and sampling approaches to allow us to advance heterocorrelated mass spectrometry imaging (MSI) and correlated Raman microscopy (CRM) from bacterial cultures and biofilms. We then exploited these measurement advances in heterocorrelated MS/CRM imaging to determine relationship of signaling molecules and excreted signaling molecules produced by P. aeruginosa to conditions relevant to the rhizosphere. In particular, we: (1) developed a laboratory testbed mimic for the rhizosphere to enable microbial growth on slides under controlled conditions; (2) integrated specific measurements of (a) rhamnolipids, (b) quinolone/quinolones, and (c) phenazines specific to P. aeruginosa; and (3) utilized the imaging tools to probe how messenger secretion, quorum sensing and swarming behavior are correlated with behavior.

Research Organization:: Univ. of Notre Dame, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

DOE Contract Number:: SC0006642

OSTI ID:: 1235677

Report Number(s):: Final Report - Notre Dame - SC0006642

Country of Publication:: United States

Language:: English

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In Situ Correlated Molecular Imaging of Chemically Communicating Microbial Communities

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