Nitric oxide is an obligate bacterial nitrification intermediate produced by hydroxylamine oxidoreductase

Caranto, Jonathan D.; Lancaster, Kyle M.

doi:10.1073/pnas.1704504114

Nitric oxide is an obligate bacterial nitrification intermediate produced by hydroxylamine oxidoreductase

Journal Article · Mon Jul 17 00:00:00 EDT 2017 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1704504114· OSTI ID:1371603

Caranto, Jonathan D. ^[1]; ^[1]

Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853

Significance

The enzymatic reactions that occur during nitrification are nature’s means to use ammonia as cellular fuel. Complete understanding of nitrification and related processes are vital to sustainable agriculture and renewable energy technologies. The prevailing view of the first phase of nitrification is that ammonia oxidizing bacteria use two enzymes, ammonia monooxygenase and hydroxylamine oxidoreductase, to oxidize ammonia to nitrite via hydroxylamine as an obligate intermediate. Our work reveals nitric oxide as an additional obligate intermediate. The presented findings necessitate revision of a key biogeochemical process, identify a new bioenergetic role for nitric oxide, predict participation of a third enzyme in the biological oxidation of ammonia to nitrite, and will inform models toward sustainable agriculture.

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Research Organization:: Cornell Univ., Ithaca, NY (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0013997

OSTI ID:: 1371603

Alternate ID(s):: OSTI ID: 1465961

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 31 Vol. 114; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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bioinorganic chemistry
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