Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Nitric oxide: an intermediate in nitrate reduction in Klebsiella pneumoniae

Journal Article · · Am. Soc. Microbiol., Abstr. Annu. Meet.; (United States)
OSTI ID:6169402
When K. pneumoniae cells were grown anaerobically with nitrate as the final electron acceptor, there was a rapid reduction of nitrate to nitrite. The latter was further reduced to hydroxylamine and finally to ammonia. Nitrate, nitrite and nitric oxide, but not nitrous oxide, could accept electrons from the respiratory chain. During growth of the organism it was possible to trap nitric oxide with alkaline permanganate. The trapped gas represented only a small portion of the reduced electron acceptor. It would appear that a major portion of nitric oxide produced from nitrite reduction must be converted to an unknown nitrogenous intermediate with an oxidation state of +1 before its reduction to hydroxylamine. The possible nature of this elusive intermediate should be discussed.
Research Organization:
Univ. of Kentucky, Lexington
OSTI ID:
6169402
Journal Information:
Am. Soc. Microbiol., Abstr. Annu. Meet.; (United States), Journal Name: Am. Soc. Microbiol., Abstr. Annu. Meet.; (United States) Vol. 77; ISSN ASMAC
Country of Publication:
United States
Language:
English