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Title: Transgenic ferritin overproduction enhances thermochemical pretreatments in Arabidopsis

Journal Article · · Biomass and Bioenergy
 [1];  [2];  [1];  [1];  [2];  [3];  [4];  [5];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. Purdue Univ., West Lafayette, IN (United States)
  3. Purdue Univ., West Lafayette, IN (United States) ; Univ. of Maryland, College Park, MD (United States)
  4. Purdue Univ., West Lafayette, IN (United States) ; Univ. of Maryland, College Park, MD (United States)
  5. National Renewable Energy Lab. (NREL), Golden, CO (United States); Michigan State Univ., East Lansing, MI (United States)
+ Show Author Affiliations

Reducing the severity of thermochemical pretreatment by incorporating iron ions as co-catalysts has been shown to enhance the sugar yield from dilute acid pretreatments and enzymatic saccharification. However, current approach of soaking iron containing acid solutions onto milled biomass prior to pretreatment is time-consuming and subject to diffusion limitations. In this work, we overexpressed soybean ferritin protein intracellularly in Arabidopsis plants (referred to as FerIN) under the control of the 35S promoter for the purpose of accumulating iron ions in Arabidopsis plants. The transgenic Arabidopsis plants accumulated iron during growth under both normal and iron-augmented watering conditions. Prussian blue staining showed punctuate staining of iron predominantly on the interior surfaces of cell lumen in FerIN plants. The harvested transgenic biomass showed enhanced pretreatability, in that it released 13–19% more glucose and xylose than empty vector control plants. The data pointed to a positive correlation between iron concentration and sugar release during pretreatment of transgenic biomass.

Research Organization:
Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio); National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
SC000997; AC36-08-GO28308
OSTI ID:
1370483
Alternate ID(s):
OSTI ID: 1250568
Journal Information:
Biomass and Bioenergy, Vol. 72, Issue C; Related Information: C3Bio partners with Purdue University (lead); Argonne National Laboratory; National Renewable Energy Laboratory; Northeastern University; University of Tennessee; ISSN 0961-9534
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 13 works
Citation information provided by
Web of Science

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  • Journal of the American Chemical Society, Vol. 130, Issue 25 https://doi.org/10.1021/ja800492z
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Cited By (7)

Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase journal June 2017
Biomass recalcitrance: a multi-scale, multi-factor, and conversion-specific property: Fig. 1. journal June 2015
Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum) journal December 2017
Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion journal October 2016
Kinetic Modelling and Experimental Studies for the Effects of Fe2+ Ions on Xylan Hydrolysis with Dilute-Acid Pretreatment and Subsequent Enzymatic Hydrolysis journal January 2018
Identifying the ionically bound cell wall and intracellular glycoside hydrolases in late growth stage Arabidopsis stems: implications for the genetic engineering of bioenergy crops journal May 2015
Burkholderia phytofirmans Inoculation-Induced Changes on the Shoot Cell Anatomy and Iron Accumulation Reveal Novel Components of Arabidopsis-Endophyte Interaction that Can Benefit Downstream Biomass Deconstruction journal January 2016

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Ferritin
Iron accumulation
Transgenic Arabidopsis
Biomass saccharification
Dilute acid pretreatment
Prussian blue staining