Iron incorporation both intra- and extra-cellularly improves the yield and saccharification of switchgrass (Panicum virgatum L.) biomass

Lin, Chien-Yuan; Donohoe, Bryon S.; Bomble, Yannick J.; Yang, Haibing; Yunes, Manal; Sarai, Nicholas S.; Shollenberger, Todd; Decker, Stephen R.; Chen, Xiaowen; McCann, Maureen C.; Tucker, Melvin P.; Wei, Hui; Himmel, Michael E.

doi:10.1186/s13068-021-01891-4

Title: Iron incorporation both intra- and extra-cellularly improves the yield and saccharification of switchgrass (Panicum virgatum L.) biomass

Journal Article · Thu Mar 04 00:00:00 EST 2021 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-021-01891-4· OSTI ID:1769214

;

; Bomble, Yannick J.; Yang, Haibing; Yunes, Manal; Sarai, Nicholas S.; Shollenberger, Todd; Decker, Stephen R.; Chen, Xiaowen; McCann, Maureen C.; Tucker, Melvin P.; Wei, Hui; Himmel, Michael E.

Abstract Background Pretreatments are commonly used to facilitate the deconstruction of lignocellulosic biomass to its component sugars and aromatics. Previously, we showed that iron ions can be used as co-catalysts to reduce the severity of dilute acid pretreatment of biomass. Transgenic iron-accumulating Arabidopsis and rice plants exhibited higher iron content in grains, increased biomass yield, and importantly, enhanced sugar release from the biomass. Results In this study, we used intracellular ferritin (FerIN) alone and in combination with an improved version of cell wall-bound carbohydrate-binding module fused iron-binding peptide (IBPex) specifically targeting switchgrass, a bioenergy crop species. The FerIN switchgrass improved by 15% in height and 65% in yield, whereas the FerIN/IBPex transgenics showed enhancement up to 30% in height and 115% in yield. The FerIN and FerIN/IBPex switchgrass had 27% and 51% higher in planta iron accumulation than the empty vector (EV) control, respectively, under normal growth conditions. Improved pretreatability was observed in FerIN switchgrass (~ 14% more glucose release than the EV), and the FerIN/IBPex plants showed further enhancement in glucose release up to 24%. Conclusions We conclude that this iron-accumulating strategy can be transferred from model plants and applied to bioenergy crops, such as switchgrass. The intra- and extra-cellular iron incorporation approach improves biomass pretreatability and digestibility, providing upgraded feedstocks for the production of biofuels and bioproducts.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0000997; AC36-08GO28308

OSTI ID:: 1769214

Alternate ID(s):: OSTI ID: 1772444

Report Number(s):: NREL-JA-2700-79392; 55; PII: 1891

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 14 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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