Recalcitrant carbohydrates after enzymatic hydrolysis of pretreated lignocellulosic biomass

Alcantara, Maria Angeles Bermudez; Dobruchowska, Justyna; Azadi, Parastoo; Garcia, Bruno Diez; Molina-Heredia, Fernando P.; Reyes-Sosa, Francisco Manuel

doi:10.1186/s13068-016-0629-4

Recalcitrant carbohydrates after enzymatic hydrolysis of pretreated lignocellulosic biomass

Journal Article · Thu Oct 06 04:00:00 EDT 2016 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-016-0629-4· OSTI ID:1364046

Alcantara, Maria Angeles Bermudez ^[1]; Dobruchowska, Justyna ^[2]; Azadi, Parastoo ^[2]; Garcia, Bruno Diez ^[3]; Molina-Heredia, Fernando P. ^[4]; Reyes-Sosa, Francisco Manuel ^[3]

C/Energia Solar, Seville (Spain); Analytical Services and Training, University of Georgia, USA
Univ. of Georgia, Athens, GA (United States)
C/Energia Solar, Seville (Spain)
Univ. de Sevilla y CSIC, Seville (Spain)

To reduce the cost of the enzymes for the hydrolysis of lignocellulosic biomass, two main strategies have been followed: one, the reduction of enzyme dosing by the use of more efficient and stable enzymatic cocktails; another, to include accessory enzymes in the cocktails to increase yields by reducing the recalcitrant carbohydrate fraction remaining at the end of the process. To guide this second strategy, we have explored the chemical bond composition of different fractions of recalcitrant carbohydrates after enzymatic hydrolysis. As a result, two lignocellulosic feedstocks of relevance for the biofuels industry have been analyzed, corn stover and sugarcane straw. On comparing the composition of chemical bonds of the starting pretreated material with samples after standard and forced hydrolysis (with enzyme overdosing), we obtained similar sugar and chemical bond composition. In conclusion, this suggests that the current enzymatic cocktails bear the set of enzymes needed to hydrolyze these feedstocks. From our point of view, the results show the need for a parallel fine-tuning of the enzymatic cocktails with the pretreatment process to maximize sugar release yield.

View Accepted Manuscript (DOE)

Research Organization:: University of Georgia Research Foundation, Inc., Athens, GA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-93ER20097

OSTI ID:: 1364046

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 9; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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