Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa

Akinosho, Hannah; Dumitrache, Alexandru; Natzke, Jace; Muchero, Wellington; Jawdy, Sara S.; Tuskan, Gerald A.; Brown, Steven D.; Ragauskas, Arthur J.

doi:10.1021/acssuschemeng.7b00449

Title: Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa

Journal Article · Wed Apr 26 00:00:00 EDT 2017 · ACS Sustainable Chemistry & Engineering

DOI:https://doi.org/10.1021/acssuschemeng.7b00449· OSTI ID:1362259

Akinosho, Hannah ^[1]; Dumitrache, Alexandru ^[2]; Natzke, Jace ^[2]; Muchero, Wellington ^[3]; Jawdy, Sara S. ^[3]; Tuskan, Gerald A. ^[3]; Brown, Steven D. ^[2];

^[4]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Georgia Inst. of Technology, Atlanta, GA (United States). School of Chemistry and Biochemistry. Renewable BioProducts Inst.
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center. Biosciences Division. UT-ORNL Joint Inst. of Biological Science
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioSciences Division. Plant Systems Biology Group
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center. Biosciences Division. UT-ORNL Joint Inst. of Biological Science; Univ. of Tennessee, Knoxville, TN (United States). Center for Renewable Carbon. Dept. of Forestry. Dept. of Chemical and Biomolecular Engineering

The bacterium Clostridium thermocellum offers a distinct and integrated approach to ethanol production through consolidated bioprocessing (CBP). The Simons’ stain technique, which assays the accessibility of lignocellulosic biomass, has been traditionally applied to fungal cellulase systems; however, its application to CBP has not been fully explored. For this reason, the structural properties of eight Populus trichocarpa with either high or low biomass densities were compared in this paper to determine bioconversion differences during separate hydrolysis and fermentation (SHF) and CBP with C. thermocellum. Simons’ staining generally identifies low density poplar as more accessible than high density poplar. Additionally, low density P. trichocarpa generally contained less Klason lignin than high density poplar. SHF and CBP treatments consistently identified BESC-7 (high density, low accessibility, low surface roughness) as a low ethanol yielding biomass and GW-9914 (low density, high accessibility, high surface roughness) as a high ethanol yielding biomass. Upon further investigation, BESC-7 also contained a high Klason lignin content (~25%), while GW-9914 had a low lignin content (~20%). Cellulose degree of polymerization (DP) measurements exhibited a weak linear correlation with accessibility (r² = 0.17). Finally, therefore, the ethanol yields were correlated with accessibility and lignin content extremes but not cellulose DP.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: Georgia Inst. of Technology, Atlanta, GA (United States); Univ. of Tennessee, Knoxville, TN (United States)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1362259

Journal Information:: ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 6; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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