Lignin Exhibits Recalcitrance-Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants

Akinosho, Hannah; Yee, Kelsey; Rodriguez, Miguel; Muchero, Wellington; Yoo, Chang Geun; Li, Mi; Thompson, Olivia; Pu, Yunqiao Joseph; Brown, Steven D.; Mielenz, Johnathan R.; Ragauskas, Arthur J.

doi:10.1002/slct.201701572

Title: Lignin Exhibits Recalcitrance-Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants

Journal Article · Wed Nov 22 00:00:00 EST 2017 · Chemistry Select

DOI:https://doi.org/10.1002/slct.201701572· OSTI ID:1410909

Akinosho, Hannah ^[1]; Yee, Kelsey ^[2];

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^[3]; Thompson, Olivia ^[2];

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^[2]; Mielenz, Johnathan R. ^[2];

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Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Georgia Inst. of Technology, Atlanta, GA (United States). Dept. of Chemistry and Biochemistry
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Univ. of Tennessee, Knoxville, TN (United States). Center for Renewable Carbon at Wildlife, and Fisheries, Dept. of Chemical and Biomolecular Engineering & Department of Forestry; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Univ. of Tennessee, Knoxville, TN (United States). Center for Renewable Carbon at Wildlife, and Fisheries, Dept. of Chemical and Biomolecular Engineering & Department of Forestry; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division

Because cellulosic ethanol production remains cost-prohibitive„ advances in consolidated bioprocessing (CBP) have been directed towards lifting this restriction. CBP reduces the need for added enzymes and can potentially slash ethanol production costs through process integration. Clostridium thermocellum, a CBP microorganism, organizes its enzymes in a multi-enzyme complex - a stark contrast to fungal enzymes. Nonetheless, recalcitrance may limit the extent of biomass deconstruction. Here in this study, six Populus were treated with C. thermocellum (ATCC 27405) and characterized to determine structural changes that resulted from CBP. The 2D HSQC NMR spectra of lignin-enriched residues revealed that higher S/G ratio (2.6) and fewer carbon-carbon interunit linkages (generally 2–5%) were present in the top performing poplar. Furthermore, cellulose degree of polymerization data suggests that C. thermocellum likely circumvents long chain cellulose, while cellulose crystallinity and hemicellulose molecular weight data do not provide a direct indication of features connected to recalcitrance. Hence, C. thermocellum is similarly impacted by the proposed lignin properties that negatively impact biomass deconstruction using fungal enzymes.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1410909

Alternate ID(s):: OSTI ID: 1410886

Journal Information:: Chemistry Select, Vol. 2, Issue 33; ISSN 2365-6549

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 3 works

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59 BASIC BIOLOGICAL SCIENCES
cellulosic ethanol
Clostridium thermocellum
lignin
Populus trichocarpa
recalcitrance

Title: Lignin Exhibits Recalcitrance-Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants

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