U.S. Department of Energy Office of Scientific and Technical Information
Title: Generate Optimized Genetic Rhythm for Enzyme Expression in Non-native systems
Software·
OSTI ID:1360676
Most amino acids are represented by more than one codon, resulting in redundancy in the genetic code. Silent codon substitutions that do not alter the amino acid sequence still have an effect on protein expression. We have developed an algorithm, GoGREEN, to enhance the expression of foreign proteins in a host organism. GoGREEN selects codons according to frequency patterns seen in the gene of interest using the codon usage table from the host organism. GoGREEN is also designed to accommodate gaps in the sequence.This software takes for input (1) the aligned protein sequences for genes the user wishes to express, (2) the codon usage table for the host organism, (3) and the DNA sequence for the target protein found in the host organism. The program will select codons based on codon usage patterns for the target DNA sequence. The program will also select codons for “gaps” found in the aligned protein sequences using the codon usage table from the host organism.
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@misc{osti_1360676,
title = {Generate Optimized Genetic Rhythm for Enzyme Expression in Non-native systems, Version 00},
author = {},
abstractNote = {Most amino acids are represented by more than one codon, resulting in redundancy in the genetic code. Silent codon substitutions that do not alter the amino acid sequence still have an effect on protein expression. We have developed an algorithm, GoGREEN, to enhance the expression of foreign proteins in a host organism. GoGREEN selects codons according to frequency patterns seen in the gene of interest using the codon usage table from the host organism. GoGREEN is also designed to accommodate gaps in the sequence.This software takes for input (1) the aligned protein sequences for genes the user wishes to express, (2) the codon usage table for the host organism, (3) and the DNA sequence for the target protein found in the host organism. The program will select codons based on codon usage patterns for the target DNA sequence. The program will also select codons for “gaps” found in the aligned protein sequences using the codon usage table from the host organism.},
doi = {},
url = {https://www.osti.gov/biblio/1360676},
year = {Thu Nov 03 00:00:00 EDT 2016},
month = {Thu Nov 03 00:00:00 EDT 2016},
note =
}
Journal Article
·
Mon Jan 01 00:00:00 EST 1990
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·OSTI ID:1360676
