Host cells and methods for production of isobutanol

Anthony, Larry Cameron; He, Hongxian; Huang, Lixuan Lisa; Okeefe, Daniel P.; Kruckeberg, Arthur Leo; Li, Yougen; Maggio-Hall, Lori Ann; McElvain, Jessica; Nelson, Mark J.; Patnaik, Ranjan; Rothman, Steven Cary

Host cells and methods for production of isobutanol

Patent · Tue Aug 23 04:00:00 EDT 2016

OSTI ID:1351895

Anthony, Larry Cameron; He, Hongxian; Huang, Lixuan Lisa; Okeefe, Daniel P.; Kruckeberg, Arthur Leo; Li, Yougen; Maggio-Hall, Lori Ann; McElvain, Jessica; Nelson, Mark J.; Patnaik, Ranjan; Rothman, Steven Cary

Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

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Research Organization:: Butamax Advanced Biofuels LLC, Wilmington, DE (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AR0000006

Assignee:: Butamax Advanced Biofuels LLC (Wilmington, DE)

Patent Number(s):: 9,422,581

Application Number:: 13/840,649

OSTI ID:: 1351895

Country of Publication:: United States

Language:: English

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Characterization of the Saccharomyces cerevisiae YMR318C (ADH6) gene product as a broad specificity NADPH-dependent alcohol dehydrogenase: relevance in aldehyde reduction Larroy, Carol; Fernandez, M. Rosario; Gonzalez, Eva Biochemical Journal, Vol. 361, Issue 1, p. 163-172 https://doi.org/10.1042/bj3610163	journal	January 2002
Complete Genomic Sequence of the Filamentous Nitrogen-fixing Cyanobacterium Anabaena sp. Strain PCC 7120 (Supplement) Kaneko, T. DNA Research, Vol. 8, Issue 5 https://doi.org/10.1093/dnares/8.5.227	journal	January 2001
Identification, Cloning, and Characterization of a Lactococcus lactis Branched-Chain -Keto Acid Decarboxylase Involved in Flavor Formation Smit, B. A.; van Hylckama Vlieg, J. E. T.; Engels, W. J. M. Applied and Environmental Microbiology, Vol. 71, Issue 1 https://doi.org/10.1128/AEM.71.1.303-311.2005	journal	January 2005
Characterization of 1-Deoxy-d-xylulose 5-Phosphate Reductoisomerase, an Enzyme Involved in Isopentenyl Diphosphate Biosynthesis, and Identification of Its Catalytic Amino Acid Residues Kuzuyama, Tomohisa; Takahashi, Shunji; Takagi, Motoki Journal of Biological Chemistry, Vol. 275, Issue 26 https://doi.org/10.1074/jbc.M001820200	journal	April 2000
Mevalonate and Nonmevalonate Pathways for the Biosynthesis of Isoprene Units Kuzuyama, Tomohisa Bioscience, Biotechnology, and Biochemistry, Vol. 66, Issue 8 https://doi.org/10.1271/bbb.66.1619	journal	January 2002
SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling Guex, Nicolas; Peitsch, Manuel C. Electrophoresis, Vol. 18, Issue 15, p. 2714-2723 https://doi.org/10.1002/elps.1150181505	journal	January 1997
An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae Dickinson, J. Richard; Harrison, Scott J.; Hewlins, Michael J. E. Journal of Biological Chemistry, Vol. 273, Issue 40, p. 25751-25756 https://doi.org/10.1074/jbc.273.40.25751	journal	October 1998
Mechanism of ketol acid reductoisomerase. Steady-state analysis and metal ion requirement Chunduru, Srinivas K.; Mrachko, Gregory T.; Calvo, K. C. Biochemistry, Vol. 28, Issue 2 https://doi.org/10.1021/bi00428a012	journal	January 1989
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast Rothstein, Rodney Guide to Yeast Genetics and Molecular Biology https://doi.org/10.1016/0076-6879(91)94022-5	book	January 1991
Enzymology, Structure, and Dynamics of Acetohydroxy Acid Isomeroreductase Dumas, Renaud; Biou, Valérie; Halgand, Frédéric Accounts of Chemical Research, Vol. 34, Issue 5 https://doi.org/10.1021/ar000082w	journal	May 2001

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