Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis
- Icahn School of Medicine at Mount Sinai, New York, NY (United States)
- Washington Univ. School of Medicine, St. Louis, MO (United States)
Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor. Previously, the 8-kDa cytoplasmic dynein light chain (LC8) was demonstrated to interact with VP35, but the functional consequences were unclear. Furthermore we demonstrate that the interaction is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. Mutational analysis demonstrates that VP35 interaction is required for the functional effects of LC8.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- National Institutes of Health (NIH)
- Grant/Contract Number:
- R01AI107056; R01AI059536; U19AI109945; U19AI109664; U19 AI070489; R01AI081914; T32-CA09547-37
- OSTI ID:
- 1351394
- Journal Information:
- Journal of Virology, Vol. 89, Issue 9; ISSN 0022-538X
- Publisher:
- American Society for MicrobiologyCopyright Statement
- Country of Publication:
- United States
- Language:
- ENGLISH
Cited by: 38 works
Citation information provided by
Web of Science
Web of Science
Similar Records
Differential Regulation of Interferon Responses by Ebola and Marburg Virus VP35 Proteins
Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis
Mutations Abrogating VP35 Interaction with Double-Stranded RNA Render Ebola Virus Avirulent in Guinea Pigs
Journal Article
·
Thu Feb 11 00:00:00 EST 2016
· Cell Reports
·
OSTI ID:1351394
+9 more
Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis
Journal Article
·
Thu Jun 08 00:00:00 EDT 2017
· Nature Communications
·
OSTI ID:1351394
+4 more
Mutations Abrogating VP35 Interaction with Double-Stranded RNA Render Ebola Virus Avirulent in Guinea Pigs
Journal Article
·
Mon Oct 11 00:00:00 EDT 2010
· J. Virol.
·
OSTI ID:1351394
+8 more