X-ray nanoprobes and diffraction-limited storage rings: opportunities and challenges of fluorescence tomography of biological specimens

Journal Article · Wed Aug 27 00:00:00 EDT 2014 · Journal of Synchrotron Radiation (Online)

DOI:https://doi.org/10.1107/S160057751401621X· OSTI ID:1351352

de Jonge, Martin D.; Ryan, Christopher G.; Jacobsen, Chris J.

X-ray nanoprobes require coherent illumination to achieve optic-limited resolution, and so will benefit directly from diffraction-limited storage rings. Here, the example of high-resolution X-ray fluorescence tomography is focused on as one of the most voracious demanders of coherent photons, since the detected signal is only a small fraction of the incident flux. Alternative schemes are considered for beam delivery, sample scanning and detectors. One must consider as well the steps before and after the X-ray experiment: sample preparation and examination conditions, and analysis complexity due to minimum dose requirements and self-absorption. By understanding the requirements and opportunities for nanoscale fluorescence tomography, one gains insight into the R&D challenges in optics and instrumentation needed to fully exploit the source advances that diffraction-limited storage rings offer.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
• Sponsoring Organization: FOREIGN
• OSTI ID: 1351352
• Journal Information: Journal of Synchrotron Radiation (Online), Vol. 21, Issue 5; ISSN 1600-5775
• Publisher: International Union of Crystallography
• Country of Publication: United States
• Language: ENGLISH