New strategies for designing inexpensive but selective bioadsorbants for environmental pollutants: Selection of specific ligands and their cell surface expression. Technical progress report, September 15, 1996--September 14, 1997

Technical Report · Wed Jan 01 04:00:00 EST 1997

DOI:https://doi.org/10.2172/13501· OSTI ID:13501

Iverson, B

'Progress for the last twelve months has revolved around setting up an antibody engineering and surface display system for use with a metal-complex binding antibody. (1) The author has isolated genes for the V regions of the heavy and light chains for the Ru(bpy)3 specific monoclonal AC1106 (Shreder, K S., Hariman, A., and Iverson, B.L., J. Am. Chem. Soc. 1996, 118, 3192-3201). This antibody binds Ru(bpy), derivatives with better than nanomolar affinity, and will serve as the generic metal-complex binding pocket. Cloning antibody genes from hybridomas is complicated by the fact that primers must be found that amplify the particular heavy and light chain genes in the hybridoma of interest. Antibody gene amplification primers are generally designed to amplify antibody repertoires from lymphocyte mRNA isolated from animals. While cloning repertoires from animals is routinely successful due to the diverse population of target m RNA, hybridomas have a single target sequence. Therefore, multiple primers and conditions must be tried before the correct primer combination is identified.'

• Research Organization: Univ. of Texas, Dept. of Chemistry and Biochemistry, Austin, TX (US)
• Sponsoring Organization: USDOE Office of Environmental Management (EM), Office of Science and Risk Policy
• DOE Contract Number: FG07-96ER62322
• OSTI ID: 13501
• Report Number(s): EMSP-55185--97; ON: DE00013501
• Country of Publication: United States
• Language: English

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