TIR-only protein RBA1 recognizes a pathogen effector to regulate cell death in Arabidopsis

Nishimura, Marc T.; Anderson, Ryan G.; Cherkis, Karen A.; Law, Terry F.; Liu, Qingli L.; Machius, Mischa; Nimchuk, Zachary L.; Yang, Li; Chung, Eui-Hwan; El Kasmi, Farid; Hyunh, Michael; Nishimura, Erin Osborne; Sondek, John E.; Dangl, Jeffery L.

doi:10.1073/pnas.1620973114

Title: TIR-only protein RBA1 recognizes a pathogen effector to regulate cell death in Arabidopsis

Journal Article · Mon Jan 30 00:00:00 EST 2017 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1620973114· OSTI ID:1349919

^[1]; Anderson, Ryan G. ^[2]; Cherkis, Karen A. ^[2]; Law, Terry F. ^[2]; Liu, Qingli L. ^[2]; Machius, Mischa ^[2]; Nimchuk, Zachary L. ^[2]; Yang, Li ^[2]; Chung, Eui-Hwan ^[2]; El Kasmi, Farid ^[2]; Hyunh, Michael ^[2];

^[1]; Sondek, John E. ^[2]; Dangl, Jeffery L. ^[2]

Univ. of North Carolina, Chapel Hill, NC (United States); Colorado State Univ., Fort Collins, CO (United States)
Univ. of North Carolina, Chapel Hill, NC (United States)

Detection of pathogens by plants is mediated by intracellular nucleotide-binding site leucine-rich repeat (NLR) receptor proteins. NLR proteins are defined by their stereotypical multidomain structure: an N-terminal Toll–interleukin receptor (TIR) or coiled-coil (CC) domain, a central nucleotide-binding (NB) domain, and a C-terminal leucine-rich repeat (LRR). The plant innate immune system contains a limited NLR repertoire that functions to recognize all potential pathogens. We isolated Response to the bacterial type III effector protein HopBA1 (RBA1), a gene that encodes a TIR-only protein lacking all other canonical NLR domains. RBA1 is sufficient to trigger cell death in response to HopBA1. We generated a crystal structure for HopBA1 and found that it has similarity to a class of proteins that includes esterases, the heme-binding protein ChaN, and an uncharacterized domain of Pasteurella multocida toxin. Self-association, coimmunoprecipitation with HopBA1, and function of RBA1 require two previously identified TIR–TIR dimerization interfaces. Although previously described as distinct in other TIR proteins, in RBA1 neither of these interfaces is sufficient when the other is disrupted. These data suggest that oligomerization of RBA1 is required for function. As a result, our identification of RBA1 demonstrates that “truncated” NLRs can function as pathogen sensors, expanding our understanding of both receptor architecture and the mechanism of activation in the plant immune system.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Science Foundation (NSF); Betty Moore Foundation

Grant/Contract Number:: IOS-1257373; GBMF3030

OSTI ID:: 1349919

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 10; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 102 works

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Diversification of defensins and NLRs in Arabidopsis species by different evolutionary mechanisms Mondragón-Palomino, Mariana; Stam, Remco; John-Arputharaj, Ajay BMC Evolutionary Biology, Vol. 17, Issue 1 https://doi.org/10.1186/s12862-017-1099-4	journal	December 2017
The bacterial Ras/Rap1 site-specific endopeptidase RRSP cleaves Ras through an atypical mechanism to disrupt Ras-ERK signaling Biancucci, Marco; Minasov, George; Banerjee, Avik Science Signaling, Vol. 11, Issue 550 https://doi.org/10.1126/scisignal.aat8335	journal	October 2018
HOS15 and HDA9 negatively regulate immunity through histone deacetylation of intracellular immune receptor NLR genes in Arabidopsis Yang, Leiyun; Chen, Xiangsong; Wang, Zhixue New Phytologist, Vol. 226, Issue 2 https://doi.org/10.1111/nph.16380	journal	January 2020
Signaling from the plasma-membrane localized plant immune receptor RPM1 requires self-association of the full-length protein El Kasmi, Farid; Chung, Eui-Hwan; Anderson, Ryan G. Proceedings of the National Academy of Sciences, Vol. 114, Issue 35 https://doi.org/10.1073/pnas.1708288114	journal	August 2017
NAD⁺ cleavage activity by animal and plant TIR domains in cell death pathways. Horsefield, Shane; Burdett, Hayden; Zhang, Xiaoxiao Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.43866	text	January 2019
Activation of a Plant NLR Complex through Heteromeric Association with an Autoimmune Risk Variant of Another NLR Tran, Diep T. N.; Chung, Eui-Hwan; Habring-Müller, Anette Current Biology, Vol. 27, Issue 8 https://doi.org/10.1016/j.cub.2017.03.018	journal	April 2017
Divergence and Conservative Evolution of XTNX Genes in Land Plants Zhang, Yan-Mei; Xue, Jia-Yu; Liu, Li-Wei Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.01844	journal	October 2017
Diversification of defensins and NLRs in Arabidopsis species by different evolutionary mechanisms Mondragón-Palomino, Mariana; Stam, Remco; John-Arputharaj, Ajay Universität Regensburg https://doi.org/10.5283/epub.36643	text	January 2017

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