Antibody profiling sensitivity through increased reporter antibody layering

Apel, William A.; Thompson, Vicki S.

Title: Antibody profiling sensitivity through increased reporter antibody layering

Patent · Tue Mar 28 00:00:00 EDT 2017

OSTI ID:1348364

Apel, William A.; Thompson, Vicki S.

A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

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Research Organization:: Idaho National Laboratory (INL), Idaho Falls, ID (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC07-99ID13727; AC07-94ID13223; AC07-05ID14517

Assignee:: Battelle Energy Alliance, LLC

Patent Number(s):: RE46,351

Application Number:: 13/771,915

OSTI ID:: 1348364

Resource Relation:: Patent File Date: 2013 Feb 20

Country of Publication:: United States

Language:: English

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