Antibody profiling sensitivity through increased reporter antibody layering

Apel, William A.; Thompson, Vicki S

Title: Antibody profiling sensitivity through increased reporter antibody layering

Patent · Tue Apr 13 00:00:00 EDT 2010

OSTI ID:1176231

Apel, William A.; Thompson, Vicki S

A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

View Patent

Cite

Export

Save

Research Organization:: Idaho National Laboratory (INL), Idaho Falls, ID (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC07-05ID14517

Assignee:: Battelle Energy Alliance, LLC (Idaho Falls, ID)

Patent Number(s):: 7,695,919

Application Number:: 11/691,096

OSTI ID:: 1176231

Country of Publication:: United States

Language:: English

References (24)

Drug Abuse Handbook Karch, Steven B. https://doi.org/10.1201/9781420048292	reference-book	December 1997
Simple and Reliable Salivary Tests for HIV and Hepatitis A and B Virus Diagnosis and Surveillance Parry, John V. Annals of the New York Academy of Sciences, Vol. 694, Issue 1 Saliva as a D https://doi.org/10.1111/j.1749-6632.1993.tb18355.x	journal	September 1993
Yeast RNA polymerase II genes: isolation with antibody probes Young, R.; Davis, R. Science, Vol. 222, Issue 4625 https://doi.org/10.1126/science.6356359	journal	November 1983
Peptides on phage: a vast library of peptides for identifying ligands Cwirla, S. E.; Peters, E. A.; Barrett, R. W. Proceedings of the National Academy of Sciences, Vol. 87, Issue 16, p. 6378-6382 https://doi.org/10.1073/pnas.87.16.6378	journal	August 1990
Testing for drugs of abuse in saliva and sweat Kidwell, David A.; Holland, Janel C.; Athanaselis, Sotiris Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 713, Issue 1, p. 111-135 https://doi.org/10.1016/S0378-4347(97)00572-0	journal	August 1998
Athletic drug Testing Bowers, Larry D. Clinics in Sports Medicine, Vol. 17, Issue 2 https://doi.org/10.1016/S0278-5919(05)70082-X	journal	April 1998
Fiber-Optic Immunosensor for the Detection of Fumonisin B₁ Thompson, Vicki S.; Maragos, Chris M. Journal of Agricultural and Food Chemistry, Vol. 44, Issue 4, p. 1041-1046 https://doi.org/10.1021/jf950661t	journal	April 1996
Combinatorial Peptide Library Protocols Cabilly, Shmuel Methods in Molecular Biology https://doi.org/10.1385/0896033929	book	January 1998
Preliminary Investigations into Tris(2,2'-bipyridyl) Ruthenium (III) as a Chemiluminescent Reagent for the Detection of 3,6-Diacetylmorphine (Heroin) on Surfaces Agg, Kent M.; Barnett, Neil W.; Lewis, Simon W. Journal of Forensic Sciences, Vol. 52, Issue 5, p. 1111-1114 https://doi.org/10.1111/j.1556-4029.2007.00424.x	journal	September 2007
Difference gel electrophoresis. A single gel method for detecting changes in protein extracts Ünlü, Mustafa; Morgan, Mary E.; Minden, Jonathan S. Electrophoresis, Vol. 18, Issue 11, p. 2071-2077 https://doi.org/10.1002/elps.1150181133	journal	January 1997
Antibody profiling as an identification tool for forensic samples Thompson, Vicki S.; Barrett, Karen B.; Davis, Tilton Enabling Technologies for Law Enforcement and Security, vp. https://doi.org/10.1117/12.334545	conference	February 1999
Electrogenerated Chemiluminescence: An Oxidative-Reduction Type ECL Reaction Sequence Using Tripropyl Amine Leland, Jonathan K.; Powell, Michael J. Journal of The Electrochemical Society, Vol. 137, Issue 10, p. 3127-3131 https://doi.org/10.1149/1.2086171	journal	January 1990
Cloning of open reading frames and promoters from the Saccharomyces cerevisiae genome: construction of genomic libraries of random small fragments Santangelo, George M.; Tornow, Joanne; Moldave, Kivie Gene, Vol. 46, Issue 2-3, p. 181-186 https://doi.org/10.1016/0378-1119(86)90402-6	journal	January 1986
Sensitivity of Two Enzyme-linked Immunosorbent Assay Tests in Relation to Western Blot in Detecting Human T-Cell Lymphotropic Virus Types I and II Infection among HIV-1 Infected Patients from São Paulo, Brazil Caterino-de-Araujo, Adele; de los Santos-Fortuna, Elizabeth; Meleiro, Mônica Cristina Zandoná Diagnostic Microbiology and Infectious Disease, Vol. 30, Issue 3, p. 173-182 https://doi.org/10.1016/S0732-8893(97)00236-8	journal	March 1998
Saliva Testing for Drugs of Abuse Cone, Edward J. Annals of the New York Academy of Sciences, Vol. 694, Issue 1, p. 91-127 https://doi.org/10.1111/j.1749-6632.1993.tb18346.x	journal	September 1993
Fluoroimmunoassays and immunofluorometric assays. Hemmilä, I. Clinical Chemistry, Vol. 31, Issue 3 https://doi.org/10.1093/clinchem/31.3.359	journal	March 1985
Drugs of Abuse in Saliva: A Review Schramm, W.; Smith, R. H.; Craig, P. A. Journal of Analytical Toxicology, Vol. 16, Issue 1, p. 1-9 https://doi.org/10.1093/jat/16.1.1	journal	January 1992
DNA SEQUENCING: Massively Parallel Genomics Fodor, S. P. Science, Vol. 277, Issue 5324 https://doi.org/10.1126/science.277.5324.393	journal	July 1997
Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale DeRisi, J. L.; Iyer, Vishwanath R.; Brown, Patrick O. Science, Vol. 278, Issue 5338, p. 680-686 https://doi.org/10.1126/science.278.5338.680	journal	October 1997
Random peptide libraries: a source of specific protein binding molecules Devlin, J.; Panganiban, L.; Devlin, P. Science, Vol. 249, Issue 4967 https://doi.org/10.1126/science.2143033	journal	July 1990
A new type of synthetic peptide library for identifying ligand-binding activity Lam, Kit S.; Salmon, Sydney E.; Hersh, Evan M. Nature, Vol. 354, Issue 6348 https://doi.org/10.1038/354082a0	journal	November 1991
Searching for peptide ligands with an epitope library Scott, J.; Smith, G. Science, Vol. 249, Issue 4967 https://doi.org/10.1126/science.1696028	journal	July 1990
Antibody Fingerprinting: A Novel Method for Identifying Individual People and Animals Francoeur, Ann-Michele Nature Biotechnology, Vol. 6, Issue 7 https://doi.org/10.1038/nbt0788-822	journal	July 1988
Micromosaic Immunoassays Bernard, André; Michel, Bruno; Delamarche, Emmanuel Analytical Chemistry, Vol. 73, Issue 1, p. 8-12 https://doi.org/10.1021/ac0008845	journal	November 2000

Similar Records

Antibody profiling sensitivity through increased reporter antibody layering

Patent · Tue Mar 28 00:00:00 EDT 2017 · OSTI ID:1176231

Apel, William A.; Thompson, Vicki S.

Antibody profiling sensitivity through increased reporter antibody layering

Patent · Tue Feb 26 00:00:00 EST 2013 · OSTI ID:1176231

Apel, William A; Thompson, Vicki S

Antibody profiling sensitivity through increased reporter antibody layering

Patent · Tue Feb 26 00:00:00 EST 2013 · OSTI ID:1176231

Apel, William A.; Thompson, Vicki S.

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
47 OTHER INSTRUMENTATION

Title: Antibody profiling sensitivity through increased reporter antibody layering

Citation Formats

References (24)

Similar Records

Related Subjects