Directed evolution of an ultrastable carbonic anhydrase for highly efficient carbon capture from flue gas

Alvizo, Oscar; Nguyen, Luan J.; Savile, Christopher K.; Bresson, Jamie A.; Lakhapatri, Satish L.; Solis, Earl O.  P.; Fox, Richard J.; Broering, James M.; Benoit, Michael R.; Zimmerman, Sabrina A.; Novick, Scott J.; Liang, Jack; Lalonde, James J.

doi:10.1073/pnas.1411461111

Title: Directed evolution of an ultrastable carbonic anhydrase for highly efficient carbon capture from flue gas

Journal Article · Mon Nov 03 00:00:00 EST 2014 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1411461111· OSTI ID:1348349

Alvizo, Oscar ^[1]; Nguyen, Luan J. ^[2]; Savile, Christopher K. ^[1]; Bresson, Jamie A. ^[1]; Lakhapatri, Satish L. ^[3]; Solis, Earl O. P. ^[2]; Fox, Richard J. ^[4]; Broering, James M. ^[5]; Benoit, Michael R. ^[1]; Zimmerman, Sabrina A. ^[6]; Novick, Scott J. ^[1]; Liang, Jack ^[1]; Lalonde, James J. ^[1]

Codexis, Inc., Redwood City, CA (United States)
Calysta Energy, Inc., Menlo Park, CA (United States)
Siluria Technologies Inc., San Francisco, CA (United Sates)
Pioneer Hi-Bred International, Inc., Johnston, IA (United States)
Novosymes Inc, Franklinton, NC (United States)
BP Biofuels, San Diego, CA (United States)

Carbonic anhydrase (CA) is one of nature’s fastest enzymes and can dramatically improve the economics of carbon capture under demanding environments such as coal-fired power plants. The use of CA to accelerate carbon capture is limited by the enzyme’s sensitivity to the harsh process conditions. Using directed evolution, the properties of a β-class CA from Desulfovibrio vulgaris were dramatically enhanced. Iterative rounds of library design, library generation, and high-throughput screening identified highly stable CA variants that tolerate temperatures of up to 107 °C in the presence of 4.2 M alkaline amine solvent at pH >10.0. This increase in thermostability and alkali tolerance translates to a 4,000,000-fold improvement over the natural enzyme. In conclusion, at pilot scale, the evolved catalyst enhanced the rate of CO2 absorption 25-fold compared with the noncatalyzed reaction.

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Research Organization:: Codexis, Inc., Redwood City, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AR0000071

OSTI ID:: 1348349

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 46; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 114 works

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