Carbonic Anhydrase Robustness for Use in Nanoscale CO 2 Capture Technologies

Sharma, Arjun; Chiang, Rong-an; Manginell, Monica; Nardi, Isaac; Coker, Eric N.; Vanegas, Juan M.; Rempe, Susan B.; Bachand, George D.

doi:10.1021/acsomega.3c02630

Title: Carbonic Anhydrase Robustness for Use in Nanoscale CO ₂ Capture Technologies

Journal Article · Thu Oct 05 00:00:00 EDT 2023 · ACS Omega

DOI:https://doi.org/10.1021/acsomega.3c02630· OSTI ID:2007674

Sharma, Arjun ^[1]; Chiang, Rong-an ^[2]; Manginell, Monica ^[3]; Nardi, Isaac ^[4];

^[5];

^[1];

^[3];

^[3]

Department of Physics, The University of Vermont, Burlington, Vermont 05405-0160, United States
Memzyme, LLC, Albuquerque, New Mexico 87123, United States
Center for Integrated Nanotechnologies, Sandia National Laboratories, Albuquerque, New Mexico 87185, United States
Epigentor Consultants, Inc., Miami, Florida 87185, United States
Electronic, Optical, and Nanomaterials Department, Sandia National Laboratories, Albuquerque, New Mexico 87185, United States

Continued dependence on crude oil and natural gas resources for fossil fuels has caused global atmospheric carbon dioxide (CO₂) emissions to increase to record-setting proportions. There is an urgent need for efficient and inexpensive carbon sequestration systems to mitigate large-scale emissions of CO₂ from industrial flue gas. Carbonic anhydrase (CA) has shown high potential for enhanced CO₂ capture applications compared to conventional absorption-based methods currently utilized in various industrial settings. This study aims to understand structural aspects that contribute to the stability of CA enzymes critical for their applications in industrial processes, which require the ability to withstand conditions different from those in their native environments. Here, we evaluated the thermostability and enzyme activity of mesophilic and thermophilic CA variants at different temperature conditions and in the presence of atmospheric gas pollutants like nitrogen oxides and sulfur oxides. Based on our enzyme activity assays and molecular dynamics simulations, we see increased conformational stability and CA activity levels in thermostable CA variants incubated week-long at different temperature conditions. The thermostable CA variants also retained high levels of CA activity despite changes in solution pH due to increasing NO and SO₂ concentrations. A loss of CA activity was observed only at high concentrations of NO/SO₂ that possibly can be minimized with the appropriate buffered solutions.

View Journal Article

Cite

Export

Save

Research Organization:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: TCF-17-13314; NA0003525

OSTI ID:: 2007674

Alternate ID(s):: OSTI ID: 2202582; OSTI ID: 2320334

Report Number(s):: SAND-2024-02785J

Journal Information:: ACS Omega, Journal Name: ACS Omega Vol. 8 Journal Issue: 41; ISSN 2470-1343

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

References (81)

Recent developments on membranes for post-combustion carbon capture Ramasubramanian, Kartik; Ho, WS Winston Current Opinion in Chemical Engineering, Vol. 1, Issue 1 https://doi.org/10.1016/j.coche.2011.08.002	journal	October 2011
Immobilization of carbonic anhydrase for CO2 capture and its industrial implementation: A review Molina-Fernández, Cristhian; Luis, Patricia Journal of CO2 Utilization, Vol. 47 https://doi.org/10.1016/j.jcou.2021.101475	journal	May 2021
Improved Treatment of Ligands and Coupling Effects in Empirical Calculation and Rationalization of p K _a Values Søndergaard, Chresten R.; Olsson, Mats H. M.; Rostkowski, Michał Journal of Chemical Theory and Computation, Vol. 7, Issue 7 https://doi.org/10.1021/ct200133y	journal	June 2011
Gas Separation Membrane Materials: A Perspective Baker, Richard W.; Low, Bee Ting Macromolecules, Vol. 47, Issue 20 https://doi.org/10.1021/ma501488s	journal	September 2014
In situ layer-by-layer assembled carbonic anhydrase-coated hollow fiber membrane contactor for rapid CO2 absorption Yong, Joel K. J.; Stevens, Geoffrey W.; Caruso, Frank Journal of Membrane Science, Vol. 514 https://doi.org/10.1016/j.memsci.2016.05.020	journal	September 2016
Biochemical properties of a novel and highly thermostable bacterial α-carbonic anhydrase fromSulfurihydrogenibium yellowstonense YO3AOP1 Capasso, Clemente; De Luca, Viviana; Carginale, Vincenzo Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 27, Issue 6 https://doi.org/10.3109/14756366.2012.703185	journal	July 2012
Water Networks in Fast Proton Transfer during Catalysis by Human Carbonic Anhydrase II Mikulski, Rose; West, Dayne; Sippel, Katherine H. Biochemistry, Vol. 52, Issue 1 https://doi.org/10.1021/bi301099k	journal	December 2012
Analysis and optimal design of membrane processes for flue gas CO2 capture Li, Qinghua; Wu, Hongyu; Wang, Zhi Separation and Purification Technology, Vol. 298 https://doi.org/10.1016/j.seppur.2022.121584	journal	October 2022
Crosslinked on novel nanofibers with thermophilic carbonic anhydrase for carbon dioxide sequestration Effendi, Sefli Sri Wahyu; Chiu, Chen-Yaw; Chang, Yu-Kaung International Journal of Biological Macromolecules, Vol. 152 https://doi.org/10.1016/j.ijbiomac.2019.11.234	journal	June 2020
The effect of net charge on the solubility, activity, and stability of ribonuclease Sa Shaw, Kevin L.; Grimsley, Gerald R.; Yakovlev, Gennady I. Protein Science, Vol. 10, Issue 6 https://doi.org/10.1110/ps.440101	journal	June 2001
Pressure control using stochastic cell rescaling Bernetti, Mattia; Bussi, Giovanni The Journal of Chemical Physics, Vol. 153, Issue 11 https://doi.org/10.1063/5.0020514	journal	September 2020
Bacterial extremo-α-carbonic anhydrases from deep-sea hydrothermal vents as potential biocatalysts for CO2 sequestration Jo, Byung Hoon; Seo, Jeong Hyun; Cha, Hyung Joon Journal of Molecular Catalysis B: Enzymatic, Vol. 109 https://doi.org/10.1016/j.molcatb.2014.08.002	journal	November 2014
The resilience of carbonic anhydrase enzyme for membrane-based carbon capture applications Yong, Joel K. J.; Stevens, Geoffrey W.; Caruso, Frank International Journal of Greenhouse Gas Control, Vol. 62 https://doi.org/10.1016/j.ijggc.2017.04.006	journal	July 2017
Improved side-chain torsion potentials for the Amber ff99SB protein force field Lindorff-Larsen, Kresten; Piana, Stefano; Palmo, Kim Proteins: Structure, Function, and Bioinformatics https://doi.org/10.1002/prot.22711	journal	January 2010
Purification and Characterization of Carbonic Anhydrase from Bovine Erythrocyte Plasma Membrane Demir, Yazar; Demir, Nazan; Nadaroglu, Hayrunnisa Preparative Biochemistry and Biotechnology, Vol. 30, Issue 1 https://doi.org/10.1080/10826060008544944	journal	January 2000
Polymeric Gas-Separation Membranes for Petroleum Refining Alqaheem, Yousef; Alomair, Abdulaziz; Vinoba, Mari International Journal of Polymer Science, Vol. 2017 https://doi.org/10.1155/2017/4250927	journal	January 2017
Entrapment of Enzymes within Sol–Gel-Derived Magnetite Drozdov, Andrey S.; Shapovalova, Olga E.; Ivanovski, Vladimir Chemistry of Materials, Vol. 28, Issue 7 https://doi.org/10.1021/acs.chemmater.6b00193	journal	March 2016
CO2-Alkanolamine Reaction Kinetics: A Review of Recent Studies Vaidya, P. D.; Kenig, E. Y. Chemical Engineering & Technology, Vol. 30, Issue 11 https://doi.org/10.1002/ceat.200700268	journal	November 2007
Thermal Inactivation and Conformational Lock of Bovine Carbonic Anhydrase Alaei, L.; A. Moosavi-Movahedi, A.; Hadi, H. Protein & Peptide Letters, Vol. 19, Issue 8 https://doi.org/10.2174/092986612801619507	journal	July 2012
Membrane-based carbon capture from flue gas: a review Khalilpour, Rajab; Mumford, Kathryn; Zhai, Haibo Journal of Cleaner Production, Vol. 103 https://doi.org/10.1016/j.jclepro.2014.10.050	journal	September 2015
Thermal stability of carbonic anhydrase immobilized within polyurethane foam Kanbar, Bora; Ozdemir, Ekrem Biotechnology Progress, Vol. 26, Issue 5 https://doi.org/10.1002/btpr.452	journal	April 2010
Comparison of simple potential functions for simulating liquid water Jorgensen, William L.; Chandrasekhar, Jayaraman; Madura, Jeffry D. The Journal of Chemical Physics, Vol. 79, Issue 2 https://doi.org/10.1063/1.445869	journal	July 1983
Structural annotation of human carbonic anhydrases Aggarwal, Mayank; Boone, Christopher D.; Kondeti, Bhargav Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 28, Issue 2 https://doi.org/10.3109/14756366.2012.737323	journal	November 2012
GROMACS: High performance molecular simulations through multi-level parallelism from laptops to supercomputers Abraham, Mark James; Murtola, Teemu; Schulz, Roland SoftwareX, Vol. 1-2 https://doi.org/10.1016/j.softx.2015.06.001	journal	September 2015
An α-carbonic anhydrase from the thermophilic bacterium Sulphurihydrogenibium azorense is the fastest enzyme known for the CO2 hydration reaction Luca, Viviana De; Vullo, Daniela; Scozzafava, Andrea Bioorganic & Medicinal Chemistry, Vol. 21, Issue 6 https://doi.org/10.1016/j.bmc.2012.09.047	journal	March 2013
Comparison of multiple Amber force fields and development of improved protein backbone parameters Hornak, Viktor; Abel, Robert; Okur, Asim Proteins: Structure, Function, and Bioinformatics, Vol. 65, Issue 3 https://doi.org/10.1002/prot.21123	journal	November 2006
Pollutants Emission Characteristics of an Ultra-low Coal-fired Power Unit Bing, Li; Yongqiang, Song; Qilong, Zhang IOP Conference Series: Earth and Environmental Science, Vol. 159 https://doi.org/10.1088/1755-1315/159/1/012014	journal	June 2018
Upgrading and Validation of the AMBER Force Field for Histidine and Cysteine Zinc(II)-Binding Residues in Sites with Four Protein Ligands Macchiagodena, Marina; Pagliai, Marco; Andreini, Claudia Journal of Chemical Information and Modeling, Vol. 59, Issue 9 https://doi.org/10.1021/acs.jcim.9b00407	journal	August 2019
Carbonic Anhydrase I Inhibition By Nitric Oxide: Implications For Mediation Of The Hypercapnia‐Induced Vasodilator Response Puscas, Ioan; Coltau, Marcela; Domuta, Gabriela Clinical and Experimental Pharmacology and Physiology, Vol. 27, Issue 1-2 https://doi.org/10.1046/j.1440-1681.2000.03212.x	journal	January 2000
CO ₂ solvation free energy using quasi-chemical theory Jiao, Dian; Rempe, Susan B. The Journal of Chemical Physics, Vol. 134, Issue 22 https://doi.org/10.1063/1.3598470	journal	June 2011
Carbonic anhydrase membranes for carbon capture and storage Zhang, Yiming; Zhu, Junyong; Hou, Jingwei Journal of Membrane Science Letters, Vol. 2, Issue 2 https://doi.org/10.1016/j.memlet.2022.100031	journal	November 2022
Post-combustion CO2 capture with membrane process: Practical membrane performance and appropriate pressure Xu, Jiayou; Wang, Zhi; Qiao, Zhihua Journal of Membrane Science, Vol. 581 https://doi.org/10.1016/j.memsci.2019.03.052	journal	July 2019
Carbonic Anhydrases: Versatile and Useful Biocatalysts in Chemistry and Biochemistry Angeli, Andrea; Carta, Fabrizio; Supuran, Claudiu T. Catalysts, Vol. 10, Issue 9 https://doi.org/10.3390/catal10091008	journal	September 2020
Carbonic anhydrase inhibitors: Inhibition studies of a coral secretory isoform with inorganic anions Bertucci, Anthony; Innocenti, Alessio; Zoccola, Didier Bioorganic & Medicinal Chemistry Letters, Vol. 19, Issue 3 https://doi.org/10.1016/j.bmcl.2008.12.056	journal	February 2009
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Carbonic Anhydrase as a Model for Biophysical and Physical-Organic Studies of Proteins and Protein−Ligand Binding Krishnamurthy, Vijay M.; Kaufman, George K.; Urbach, Adam R. Chemical Reviews, Vol. 108, Issue 3 https://doi.org/10.1021/cr050262p	journal	March 2008
Preparation of titania based biocatalytic nanoparticles and membranes for CO₂conversion Hou, Jingwei; Dong, Guangxi; Xiao, Bowen Journal of Materials Chemistry A, Vol. 3, Issue 7 https://doi.org/10.1039/C4TA05760K	journal	January 2015
Tracking solvent and protein movement during CO ₂ release in carbonic anhydrase II crystals Kim, Chae Un; Song, HyoJin; Avvaru, Balendu Sankara Proceedings of the National Academy of Sciences, Vol. 113, Issue 19 https://doi.org/10.1073/pnas.1520786113	journal	April 2016
Comparison of Carbonic Anhydrases for CO2 Sequestration Steger, Franziska; Reich, Johanna; Fuchs, Werner International Journal of Molecular Sciences, Vol. 23, Issue 2 https://doi.org/10.3390/ijms23020957	journal	January 2022
PROPKA3: Consistent Treatment of Internal and Surface Residues in Empirical p K _a Predictions Olsson, Mats H. M.; Søndergaard, Chresten R.; Rostkowski, Michal Journal of Chemical Theory and Computation, Vol. 7, Issue 2 https://doi.org/10.1021/ct100578z	journal	December 2010
Combined Density Functional Theory (DFT) and Continuum Calculations of p K _a in Carbonic Anhydrase Jiao, Dian; Rempe, Susan B. Biochemistry, Vol. 51, Issue 30 https://doi.org/10.1021/bi201771q	journal	July 2012
Canonical sampling through velocity rescaling Bussi, Giovanni; Donadio, Davide; Parrinello, Michele The Journal of Chemical Physics, Vol. 126, Issue 1 https://doi.org/10.1063/1.2408420	journal	January 2007
Consumption of carbonyl sulphide (COS) by higher plant carbonic anhydrase (CA) Protoschill-Krebs, G.; Wilhelm, C.; Kesselmeier, J. Atmospheric Environment, Vol. 30, Issue 18 https://doi.org/10.1016/1352-2310(96)00026-X	journal	September 1996
Novel alkalistable α-carbonic anhydrase from the polyextremophilic bacterium Bacillus halodurans: characteristics and applicability in flue gas CO2 sequestration Faridi, Shazia; Satyanarayana, T. Environmental Science and Pollution Research, Vol. 23, Issue 15 https://doi.org/10.1007/s11356-016-6642-0	journal	April 2016
LINCS: A linear constraint solver for molecular simulations Hess, Berk; Bekker, Henk; Berendsen, Herman J. C. Journal of Computational Chemistry, Vol. 18, Issue 12 https://doi.org/10.1002/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H	journal	September 1997
Upgraded AMBER Force Field for Zinc-Binding Residues and Ligands for Predicting Structural Properties and Binding Affinities in Zinc-Proteins Macchiagodena, Marina; Pagliai, Marco; Andreini, Claudia ACS Omega, Vol. 5, Issue 25 https://doi.org/10.1021/acsomega.0c01337	journal	June 2020
Stable Carbon Cycle-Climate Relationship During the Late Pleistocene Siegenthaler, U. Science, Vol. 310, Issue 5752 https://doi.org/10.1126/science.1120130	journal	November 2005
Demonstration Results of Enzyme-Accelerated CO2 Capture Fradette, Louis; Lefebvre, Sylvain; Carley, Jonathan Energy Procedia, Vol. 114 https://doi.org/10.1016/j.egypro.2017.03.1263	journal	July 2017
Structural and functional changes of bovine carbonic anhydrase as a consequence of temperature. Sarraf, N. S.; Saboury, A. A.; Ranjbar, B. Acta Biochimica Polonica, Vol. 51, Issue 3 https://doi.org/10.18388/abp.2004_3551	journal	September 2004
Atmospheric Methane and Nitrous Oxide of the Late Pleistocene from Antarctic Ice Cores Spahni, R. Science, Vol. 310, Issue 5752 https://doi.org/10.1126/science.1120132	journal	November 2005
MOF based CO2 capture: Adsorption and membrane separation Zhao, Meng; Yang, Yun; Gu, Xue-Song Inorganic Chemistry Communications, Vol. 152 https://doi.org/10.1016/j.inoche.2023.110722	journal	June 2023
Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical features Kabsch, Wolfgang; Sander, Christian Biopolymers, Vol. 22, Issue 12 https://doi.org/10.1002/bip.360221211	journal	December 1983
Development of Integrated System for Biomimetic CO ₂ Sequestration Using the Enzyme Carbonic Anhydrase Bond, Gillian M.; Stringer, John; Brandvold, Donald K. Energy & Fuels, Vol. 15, Issue 2 https://doi.org/10.1021/ef000246p	journal	March 2001
Climate and atmospheric history of the past 420,000 years from the Vostok ice core, Antarctica Petit, J. R.; Jouzel, J.; Raynaud, D. Nature, Vol. 399, Issue 6735 https://doi.org/10.1038/20859	journal	June 1999
Ultra-thin enzymatic liquid membrane for CO2 separation and capture Fu, Yaqin; Jiang, Ying-Bing; Dunphy, Darren Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-03285-x	journal	March 2018
Protein Adsorption Orientation in the Light of Fluorescent Probes: Mapping of the Interaction between Site-Directly Labeled Human Carbonic Anhydrase II and Silica Nanoparticles Karlsson, Martin; Carlsson, Uno Biophysical Journal, Vol. 88, Issue 5 https://doi.org/10.1529/biophysj.104.054809	journal	May 2005
A review of material development in the field of carbon capture and the application of membrane-based processes in power plants and energy-intensive industries He, Xuezhong Energy, Sustainability and Society, Vol. 8, Issue 1 https://doi.org/10.1186/s13705-018-0177-9	journal	November 2018
Accuracy and efficiency of the particle mesh Ewald method Petersen, Henrik G. The Journal of Chemical Physics, Vol. 103, Issue 9 https://doi.org/10.1063/1.470043	journal	September 1995
The cost of CO2 capture and storage Rubin, Edward S.; Davison, John E.; Herzog, Howard J. International Journal of Greenhouse Gas Control, Vol. 40 https://doi.org/10.1016/j.ijggc.2015.05.018	journal	September 2015
Post-combustion carbon capture by membrane separation, Review Kárászová, Magda; Zach, Boleslav; Petrusová, Zuzana Separation and Purification Technology, Vol. 238 https://doi.org/10.1016/j.seppur.2019.116448	journal	May 2020
Biocatalytic gas-liquid membrane contactors for CO2 hydration with immobilized carbonic anhydrase Hou, Jingwei; Zulkifli, Muhammad Yazid; Mohammad, Munirah Journal of Membrane Science, Vol. 520 https://doi.org/10.1016/j.memsci.2016.07.003	journal	December 2016
Membrane-based carbon capture: Recent progress, challenges, and their role in achieving the sustainable development goals Olabi, A. G.; Alami, Abdul Hai; Ayoub, Mohamad Chemosphere, Vol. 320 https://doi.org/10.1016/j.chemosphere.2023.137996	journal	April 2023
Supported ionic liquid membranes immobilized with carbonic anhydrases for CO2 transport at high temperatures Abdelrahim, M. Yahia M.; Martins, Carla F.; Neves, Luísa. A. Journal of Membrane Science, Vol. 528 https://doi.org/10.1016/j.memsci.2017.01.033	journal	April 2017
Increased thermal stability of the carbonic anhydrase enzyme complex for the efficient reduction of CO2 through cyclization and polymerization by peptide bonding Sun, Habin; Han, JooHee; Jo, YeWon Process Biochemistry, Vol. 120 https://doi.org/10.1016/j.procbio.2022.06.001	journal	September 2022
Identification of the first small-molecule ligand of the neuronal receptor sortilin and structure determination of the receptor–ligand complex Andersen, Jacob Lauwring; Schrøder, Tenna Juul; Christensen, Søren Acta Crystallographica Section D Biological Crystallography, Vol. 70, Issue 2 https://doi.org/10.1107/S1399004713030149	journal	January 2014
Proton transport in carbonic anhydrase: Insights from molecular simulation Maupin, C. Mark; Voth, Gregory A. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1804, Issue 2 https://doi.org/10.1016/j.bbapap.2009.09.006	journal	February 2010
Power plant post-combustion carbon dioxide capture: An opportunity for membranes Merkel, Tim C.; Lin, Haiqing; Wei, Xiaotong Journal of Membrane Science, Vol. 359, Issue 1-2, p. 126-139 https://doi.org/10.1016/j.memsci.2009.10.041	journal	September 2010
Membrane Separation Processes and Post-Combustion Carbon Capture: State of the Art and Prospects Favre, Eric Membranes, Vol. 12, Issue 9 https://doi.org/10.3390/membranes12090884	journal	September 2022
Highly thermostable carbonic anhydrase from Persephonella marina EX-H1: Its expression and characterization for CO2-sequestration applications Kanth, Bashistha Kumar; Jun, So-Young; Kumari, Shipra Process Biochemistry, Vol. 49, Issue 12 https://doi.org/10.1016/j.procbio.2014.10.011	journal	December 2014
Microbial Carbonic Anhydrases in Biomimetic Carbon Sequestration for Mitigating Global Warming: Prospects and Perspectives Bose, Himadri; Satyanarayana, Tulasi Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.01615	journal	August 2017
Biocatalytic PVDF composite hollow fiber membranes for CO2 removal in gas-liquid membrane contactor Xu, Yilin; Lin, Yuqing; Chew, Nick Guan Pin Journal of Membrane Science, Vol. 572 https://doi.org/10.1016/j.memsci.2018.11.043	journal	February 2019
Peptide-Induced Affinity Binding of Carbonic Anhydrase to Carbon Nanotubes Chen, Xiaoxing; Wang, Yibing; Wang, Ping Langmuir, Vol. 31, Issue 1 https://doi.org/10.1021/la504321q	journal	January 2015
Hollow fiber carbon membranes: From material to application He, Xuezhong; Hägg, May-Britt Chemical Engineering Journal, Vol. 215-216 https://doi.org/10.1016/j.cej.2012.10.051	journal	January 2013
Membrane processes and postcombustion carbon dioxide capture: Challenges and prospects Favre, Eric Chemical Engineering Journal, Vol. 171, Issue 3 https://doi.org/10.1016/j.cej.2011.01.010	journal	July 2011
Esterase activity of carbonic anhydrases serves as surrogate for selecting antibodies blocking hydratase activity Uda, Narasimha Rao; Seibert, Volker; Stenner-Liewen, Frank Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 30, Issue 6 https://doi.org/10.3109/14756366.2014.1001754	journal	March 2015
Activation and inhibition of bovine carbonic anhydrase III by dianions. Rowlett, R. S.; Gargiulo, N. J.; Santoli, F. A. Journal of Biological Chemistry, Vol. 266, Issue 2 https://doi.org/10.1016/S0021-9258(17)35263-8	journal	January 1991
Enzymatic conversion of CO2 to bicarbonate in functionalized mesoporous silica Yu, Yuehua; Chen, Baowei; Qi, Wen Microporous and Mesoporous Materials, Vol. 153 https://doi.org/10.1016/j.micromeso.2011.12.005	journal	May 2012
Comparison of CO2 Separation Efficiency from Flue Gases Based on Commonly Used Methods and Materials Ziobrowski, Zenon; Rotkegel, Adam Materials, Vol. 15, Issue 2 https://doi.org/10.3390/ma15020460	journal	January 2022
Structure and mechanism of carbonic anhydrase Lindskog, Sven Pharmacology & Therapeutics, Vol. 74, Issue 1 https://doi.org/10.1016/S0163-7258(96)00198-2	journal	January 1997
Insights Into the Role of Exposed Surface Charged Residues in the Alkali-Tolerance of GH11 Xylanase Wu, Xiuyun; Zhang, Qun; Zhang, Lanzeng Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.00872	journal	May 2020
VMD: Visual molecular dynamics Humphrey, William; Dalke, Andrew; Schulten, Klaus Journal of Molecular Graphics, Vol. 14, Issue 1 https://doi.org/10.1016/0263-7855(96)00018-5	journal	February 1996

Similar Records

Engineered living materials for capture, conversion, and recycling technologies

Technical Report · Wed Mar 20 00:00:00 EDT 2024 · OSTI ID:2007674

Bachand, George D.; Rempe, Susan B.; Manginell, Monica; +4 more

Activity and Thermostability of GH5 Endoglucanase Chimeras from Mesophilic and Thermophilic Parents

Journal Article · Wed Feb 20 00:00:00 EST 2019 · Applied and Environmental Microbiology · OSTI ID:2007674

Zheng, Fei; Vermaas, Josh V.; Zheng, Jie; +7 more

Recovery Act: Innovative CO₂ Sequestration from Flue Gas Using Industrial Sources and Innovative Concept for Beneficial CO₂ Use

Technical Report · Tue Jul 31 00:00:00 EDT 2012 · OSTI ID:2007674

Dando, Neal; Gershenzon, Mike; Ghosh, Rajat

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Membranes
Peptides and proteins
pH
Stability
Thermal stability
membranes
peptides and proteins
stability
thermal stability

Title: Carbonic Anhydrase Robustness for Use in Nanoscale CO 2 Capture Technologies

Citation Formats

References (81)

Similar Records

Related Subjects

Title: Carbonic Anhydrase Robustness for Use in Nanoscale CO ₂ Capture Technologies