Lipid and glycolipid isomer analyses using ultra-high resolution ion mobility spectrometry separations

Wojcik, Roza; Webb, Ian K.; Deng, Liulin; Garimella, Sandilya V. B.; Prost, Spencer A.; Ibrahim, Yehia M.; Baker, Erin S.; Smith, Richard D.

doi:10.3390/ijms18010183

Lipid and glycolipid isomer analyses using ultra-high resolution ion mobility spectrometry separations

Journal Article · Wed Jan 18 00:00:00 EST 2017 · International Journal of Molecular Sciences (Online)

DOI:https://doi.org/10.3390/ijms18010183· OSTI ID:1347858

Wojcik, Roza ^[1]; Webb, Ian K. ^[1]; Deng, Liulin ^[1]; Garimella, Sandilya V. B. ^[1]; Prost, Spencer A. ^[1]; Ibrahim, Yehia M. ^[1]; Baker, Erin S. ^[1]; Smith, Richard D. ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Understanding the biological mechanisms related to lipids and glycolipids is challenging due to the vast number of possible isomers. Mass spectrometry (MS) measurements are currently the dominant approach for studying and providing detailed information on lipid and glycolipid structures. However, difficulties in distinguishing many structural isomers (e.g. distinct acyl chain positions, double bond locations, as well as glycan isomers) inhibit the understanding of their biological roles. Here we utilized ultra-high resolution ion mobility spectrometry (IMS) separations based upon the use of traveling waves in a serpentine long path length multi-pass Structures for Lossless Manipulations (SLIM) to enhance isomer resolution. The multi-pass arrangement allowed separations ranging from ~16 m (1 pass) to ~470 m (32 passes) to be investigated for the distinction of lipids and glycolipids with extremely small structural differences. Lastly, these ultra-high resolution SLIM IMS-MS analyses provide a foundation for exploring and better understanding isomer specific biological and disease processes.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States), Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1347858

Report Number(s):: PNNL-SA--122274; 48680; 48135; 453040220

Journal Information:: International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 1 Vol. 18; ISSN IJMCFK; ISSN 1422-0067

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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