Radiation damage to nucleoprotein complexes in&nbsp;macromolecular crystallography

Bury, Charles; Garman, Elspeth F.; Ginn, Helen Mary; Ravelli, Raimond B.  G.; Carmichael, Ian; Kneale, Geoff; McGeehan, John E.

doi:10.1107/s1600577514026289

Radiation damage to nucleoprotein complexes in macromolecular crystallography

Journal Article · Fri Jan 30 00:00:00 EST 2015 · Journal of Synchrotron Radiation (Online)

DOI:https://doi.org/10.1107/s1600577514026289· OSTI ID:1345569

Bury, Charles ^[1]; Garman, Elspeth F. ^[2]; Ginn, Helen Mary ^[2]; Ravelli, Raimond B. G. ^[3]; Carmichael, Ian ^[4]; Kneale, Geoff ^[5]; McGeehan, John E. ^[5]

Univ. of Oxford (United Kingdom). Dept. of Biochemistry; DOE Office of Scientific and Technical Information (OSTI)
Univ. of Oxford (United Kingdom). Dept. of Biochemistry
Maastricht Univ. (Netherlands). Inst. of Nanoscopy
Univ. of Notre Dame, IN (United States). Notre Dame Radiation Lab.
Univ. of Portsmouth (United Kingdom). Inst. of Biomedical and Biomolecular Sciences

Significant progress has been made in macromolecular crystallography over recent years in both the understanding and mitigation of X-ray induced radiation damage when collecting diffraction data from crystalline proteins. Despite the large field that is productively engaged in the study of radiation chemistry of nucleic acids, particularly of DNA, there are currently very few X-ray crystallographic studies on radiation damage mechanisms in nucleic acids. Quantitative comparison of damage to protein and DNA crystals separately is challenging, but many of the issues are circumvented by studying pre-formed biological nucleoprotein complexes where direct comparison of each component can be made under the same controlled conditions. A model protein–DNA complex C.Esp1396I is employed to investigate specific damage mechanisms for protein and DNA in a biologically relevant complex over a large dose range (2.07–44.63 MGy). In order to allow a quantitative analysis of radiation damage sites from a complex series of macromolecular diffraction data, a computational method has been developed that is generally applicable to the field. Typical specific damage was observed for both the protein on particular amino acids and for the DNA on, for example, the cleavage of base-sugar N₁—C and sugar-phosphate C—O bonds. Strikingly the DNA component was determined to be far more resistant to specific damage than the protein for the investigated dose range. We observed the protein at low doses and found that they were susceptible to radiation damage while the DNA was far more resistant, damage only being observed at significantly higher doses.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Notre Dame, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: FC02-04ER15533

OSTI ID:: 1345569

Alternate ID(s):: OSTI ID: 22437344

Journal Information:: Journal of Synchrotron Radiation (Online), Journal Name: Journal of Synchrotron Radiation (Online) Journal Issue: 2 Vol. 22; ISSN 1600-5775; ISSN JSYRES

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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