Batch crystallization of rhodopsin for structural dynamics using an X-ray free-electron laser

Wu, Wenting; Nogly, Przemyslaw; Rheinberger, Jan; Kick, Leonhard M.; Gati, Cornelius; Nelson, Garrett; Deupi, Xavier; Standfuss, Jorg; Schertler, Gebhard; Panneels, Valerie

doi:10.1107/s2053230x15009966

Batch crystallization of rhodopsin for structural dynamics using an X-ray free-electron laser

Journal Article · Sat Jun 27 00:00:00 EDT 2015 · Acta Crystallographica. Section F, Structural Biology Communications

DOI:https://doi.org/10.1107/s2053230x15009966· OSTI ID:1345038

Wu, Wenting ^[1]; Nogly, Przemyslaw ^[1]; Rheinberger, Jan ^[1]; Kick, Leonhard M. ^[1]; Gati, Cornelius ^[1]; Nelson, Garrett ^[1]; Deupi, Xavier ^[1]; Standfuss, Jorg ^[1]; Schertler, Gebhard ^[1]; Panneels, Valerie ^[1]

Paul Scherrer Inst., Villigen (Switzerland). Lab. for Biomolecular Research

Rhodopsin is a membrane protein from the G protein-coupled receptor family. Together with its ligand retinal, it forms the visual pigment responsible for night vision. In order to perform ultrafast dynamics studies, a time-resolved serial femtosecond crystallography method is required owing to the nonreversible activation of rhodopsin. In such an approach, microcrystals in suspension are delivered into the X-ray pulses of an X-ray free-electron laser (XFEL) after a precise photoactivation delay. Here in this study, a millilitre batch production of high-density microcrystals was developed by four methodical conversion steps starting from known vapour-diffusion crystallization protocols: (i) screening the low-salt crystallization conditions preferred for serial crystallography by vapour diffusion, (ii) optimization of batch crystallization, (iii) testing the crystal size and quality using second-harmonic generation (SHG) imaging and X-ray powder diffraction and (iv) production of millilitres of rhodopsin crystal suspension in batches for serial crystallography tests; these crystals diffracted at an XFEL at the Linac Coherent Light Source using a liquid-jet setup.

View Accepted Manuscript (DOE)

Research Organization:: Paul Scherrer Inst., Villigen (Switzerland). Lab. for Biomolecular Research

Sponsoring Organization:: National Inst. of Health (NIH); National Science Foundation (NSF); USDOE

OSTI ID:: 1345038

Alternate ID(s):: OSTI ID: 22389083

Journal Information:: Acta Crystallographica. Section F, Structural Biology Communications, Journal Name: Acta Crystallographica. Section F, Structural Biology Communications Journal Issue: 7 Vol. 71; ISSN ACSFEN; ISSN 2053-230X

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Perspective: Opportunities for ultrafast science at SwissFEL Abela, Rafael; Beaud, Paul; Van Bokhoven, Jeroen A. ETH Zurich https://doi.org/10.3929/ethz-b-000231826	text	January 2017
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Perspective: Opportunities for ultrafast science at SwissFEL Abela, Rafael; Beaud, Paul; van Bokhoven, Jeroen A. Structural Dynamics, Vol. 4, Issue 6 https://doi.org/10.1063/1.4997222	journal	November 2017
Linac Coherent Light Source: The first five years Bostedt, Christoph; Boutet, Sébastien; Fritz, David M. Reviews of Modern Physics, Vol. 88, Issue 1 https://doi.org/10.1103/revmodphys.88.015007	journal	March 2016
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On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan International Union of Crystallography (IUCr) https://doi.org/10.1107/s2052252519007395/mf5034sup1.pdf	text	January 2019
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s2059798318017953	journal	February 2019
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. International Union of Crystallography (IUCr) https://doi.org/10.1107/s2059798318017953/ba5296sup1.pdf	text	January 2019
Time-resolved structural studies with serial crystallography: A new light on retinal proteins Panneels, Valérie; Wu, Wenting; Tsai, Ching-Ju Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2015-04290	text	January 2015
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-02620	text	January 2019
Perspective: Opportunities for ultrafast science at SwissFEL Abela, Rafael; Beaud, Paul; Van Bokhoven, Jeroen A. AIP Publishing https://doi.org/10.7892/boris.114739	text	January 2017

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