Influence of silica matrix composition and functional component additives on the bioactivity and viability of encapsulated living cells

Savage, Travis J.; Dunphy, Darren R.; Harbaugh, Svetlana; Kelley-Loughnane, Nancy; Harper, Jason C.; Brinker, C. Jeffrey

doi:10.1021/acsbiomaterials.5b00261

Influence of silica matrix composition and functional component additives on the bioactivity and viability of encapsulated living cells

Journal Article · Fri Nov 06 00:00:00 EST 2015 · ACS Biomaterials Science & Engineering

DOI:https://doi.org/10.1021/acsbiomaterials.5b00261· OSTI ID:1340691

Savage, Travis J. ^[1]; Dunphy, Darren R. ^[1]; Harbaugh, Svetlana ^[2]; Kelley-Loughnane, Nancy ^[2]; Harper, Jason C. ^[3]; Brinker, C. Jeffrey ^[4]

Univ. of New Mexico, Albuquerque, NM (United States)
Wright Patterson Air Force Base, Dayton, OH (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Univ. of New Mexico, Albuquerque, NM (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

The remarkable impact encapsulation matrix chemistry can have on the bioactivity and viability of integrated living cells is reported. Two silica chemistries (aqueous silicate and alkoxysilane), and a functional component additive (glycerol), are employed to generate three distinct silica matrices. These matrices are used to encapsulate living E. coli cells engineered with a synthetic riboswitch for cell-based biosensing. Following encapsulation, membrane integrity, reproductive capability, and riboswitch-based protein expression levels and rates are measured over a 5 week period. Striking differences in E. coli bioactivity, viability, and biosensing performance are observed for cells encapsulated within the different matrices. E. coli cells encapsulated for 35 days in aqueous silicate-based (AqS) matrices showed relatively low membrane integrity, but high reproductive capability in comparison to cells encapsulated in glycerol containing sodium silicate-based (AqS + g) and alkoxysilane-based (PGS) gels. Further, cells in sodium silicate-based matrices showed increasing fluorescence output over time, resulting in a 1.8-fold higher fluorescence level, and a faster expression rate, over cells free in solution. Furthermore, this unusual and unique combination of biological properties demonstrates that careful design of the encapsulation matrix chemistry can improve functionality of the biocomposite material, and result in new and unexpected physiological states.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1340691

Report Number(s):: SAND--2017-0555J; 650542

Journal Information:: ACS Biomaterials Science & Engineering, Journal Name: ACS Biomaterials Science & Engineering Journal Issue: 12 Vol. 1; ISSN 2373-9878

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Influence of silica matrix composition and functional component additives on the bioactivity and viability of encapsulated living cells

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References (49)

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