Expression of a clostridial [FeFe]-hydrogenase in Chlamydomonas reinhardtii prolongs photo-production of hydrogen from water splitting

Noone, Seth; Ratcliff, Kathleen; Davis, ReAnna; Subramanian, Venkataramanan; Meuser, Jonathan; Posewitz, Matthew C.; King, Paul W.; Ghirardi, Maria L.

doi:10.1016/j.algal.2016.12.014

Expression of a clostridial [FeFe]-hydrogenase in Chlamydomonas reinhardtii prolongs photo-production of hydrogen from water splitting

Journal Article · Sat Dec 24 00:00:00 EST 2016 · Algal Research

DOI:https://doi.org/10.1016/j.algal.2016.12.014· OSTI ID:1339351

Noone, Seth ^[1]; Ratcliff, Kathleen ^[2]; Davis, ReAnna ^[1]; Subramanian, Venkataramanan ^[1]; Meuser, Jonathan ^[3]; Posewitz, Matthew C. ^[4]; King, Paul W. ^[1]; Ghirardi, Maria L. ^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States); U. S. Dept. of Energy, Washington, D.C. (United States)
Colorado School of Mines, Golden, CO (United States); Algenol, Fort Myers, FL (United States)
Colorado School of Mines, Golden, CO (United States)

The high oxygen (O₂) sensitivity of green algal [FeFe]-hydrogenases is a significant limitation for the sustained production of hydrogen gas (H₂) from photosynthetic water splitting. To address this limitation we replaced the native [FeFe]-hydrogenases with a more O₂-tolerant clostridial [FeFe]-hydrogenase CaI in Chlamydomonas reinhardtii strain D66ΔHYD (hydA1^–hydA2^–) that contains insertionally inactivated [FeFe]-hydrogenases genes. Expression and translocation of CaI in D66ΔHYD led to the recovery of H₂ photoproduction at ~ 20% of the rates of the wild-type parent strain D66. We show for the first time that a bacterial [FeFe]-hydrogenase can be expressed, localized and matured to a catalytically active form that couples to photosynthetic electron transport in the green alga C. reinhardtii. The lower rates of O₂ inactivation of CaI led to more sustained H₂ photoproduction when cultures were challenged with O₂ or kept under prolonged illumination at solar intensities. Lastly, these results provide new insights into the requisites for attaining photobiological H₂ production from water splitting using a more O₂-tolerant hydrogenase.

View Accepted Manuscript (DOE)

Research Organization:: NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))

Sponsoring Organization:: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Fuel Cell Technologies Program (EE-3F); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1339351

Alternate ID(s):: OSTI ID: 1396621

Report Number(s):: NREL/JA--2700-67527

Journal Information:: Algal Research, Journal Name: Algal Research Vol. 22; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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O ₂ sensitivity and H ₂ production activity of hydrogenases—A review Lu, Yuan; Koo, Jamin Biotechnology and Bioengineering, Vol. 116, Issue 11 https://doi.org/10.1002/bit.27136	journal	August 2019
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