Evolutionary Significance of an Algal Gene Encoding an [FeFe]-Hydrogenase with F-Domain Homology and Hydrogenase Activity in Chlorella Variabilis NC64A

Meuser, Jonathan E.; Boyd, Eric S.; Ananyev, Gennady; Karns, Devin; Radakovits, Randor; Murthy, U. M. N.; Ghirardi, Maria L.; Dismukes, G. Charles; Peters, John W.; Posewitz, Matthew C.

doi:10.1007/s00425-011-1431-y

Evolutionary Significance of an Algal Gene Encoding an [FeFe]-Hydrogenase with F-Domain Homology and Hydrogenase Activity in Chlorella Variabilis NC64A

Journal Article · Sat Oct 01 04:00:00 EDT 2011 · Planta

DOI:https://doi.org/10.1007/s00425-011-1431-y· OSTI ID:1036398

Meuser, Jonathan E.; Boyd, Eric S.; Ananyev, Gennady; Karns, Devin; Radakovits, Randor; Murthy, U. M. N.; Ghirardi, Maria L.; Dismukes, G. Charles; Peters, John W.; Posewitz, Matthew C.

[FeFe]-hydrogenases (HYDA) link the production of molecular H{sub 2} to anaerobic metabolism in many green algae. Similar to Chlamydomonas reinhardtii, Chlorella variabilis NC64A (Trebouxiophyceae, Chlorophyta) exhibits [FeFe]-hydrogenase (HYDA) activity during anoxia. In contrast to C. reinhardtii and other chlorophycean algae, which contain hydrogenases with only the HYDA active site (H-cluster), C. variabilis NC64A is the only known green alga containing HYDA genes encoding accessory FeS cluster-binding domains (F-cluster). cDNA sequencing confirmed the presence of F-cluster HYDA1 mRNA transcripts, and identified deviations from the in silico splicing models. We show that HYDA activity in C. variabilis NC64A is coupled to anoxic photosynthetic electron transport (PSII linked, as well as PSII-independent) and dark fermentation. We also show that the in vivo H{sub 2}-photoproduction activity observed is as O2 sensitive as in C. reinhardtii. The two C. variabilis NC64A HYDA sequences are similar to homologs found in more deeply branching bacteria (Thermotogales), diatoms, and heterotrophic flagellates, suggesting that an F-cluster HYDA is the ancestral enzyme in algae. Phylogenetic analysis indicates that the algal HYDA H-cluster domains are monophyletic, suggesting that they share a common origin, and evolved from a single ancestral F-cluster HYDA. Furthermore, phylogenetic reconstruction indicates that the multiple algal HYDA paralogs are the result of gene duplication events that occurred independently within each algal lineage. Collectively, comparative genomic, physiological, and phylogenetic analyses of the C. variabilis NC64A hydrogenase has provided new insights into the molecular evolution and diversity of algal [FeFe]-hydrogenases.

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22) and Joint Genome Institute; US Air Force Office of Scientific Research; NASA

DOE Contract Number:: AC36-08GO28308

OSTI ID:: 1036398

Report Number(s):: NREL/JA-2700-54258

Journal Information:: Planta, Journal Name: Planta Journal Issue: 4 Vol. 234; ISSN PLANAB; ISSN 0032-0935

Country of Publication:: United States

Language:: English

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
ALGAE
ANOXIA
BACTERIA
CHLAMYDOMONAS
CHLORELLA
DIATOMS
ELECTRONS
ENZYMES
F-cluster domain
FERMENTATION
GENES
HYDA
HYDROGENASES
IN VIVO
METABOLISM
ORIGIN
PRODUCTION
SPLICING
TRANSPORT
chlorella variablilis NC64A

Evolutionary Significance of an Algal Gene Encoding an [FeFe]-Hydrogenase with F-Domain Homology and Hydrogenase Activity in Chlorella Variabilis NC64A

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