Global DNA sequence organization in humans is vertebrate-specific but differs in nuclear and mitochondrial genomes
- Univ. of Western Ontario, London (Canada)
Although genome sequencing projects have made significant progress in the analysis of large amounts of DNA sequence information, little is known regarding primary DNA sequence organization and its determinants. Primary sequence organization was characterized for 15 different regions of the human nuclear genome (each 36 to 180 kb in length and totaling 965 kb), the complete human mitochondrial genome and several viral genomes that infect the human species. Recognition of previously unknown patterns in the organization of large DNA sequences was accomplished using chaos representation and analysis of short-sequence representation. Primary DNA sequence organization was found to have a global structure characterized by a non-random composition and order of nucleotides. Global structure was identified as a higher-order sequence organization that is independent of the functional properties and the length of the DNA sequence. Global sequence organization is similar in the human nuclear genome and viral genomes capable of integration within that genome, supporting the hypothesis of genome-type specific constraints upon primary sequence organization. Human DNA sequence organization was compared with that of four other vertebrate species and four species representing a broad phylogenetic range. Global DNA sequence organization was similar among closely-related species but different in the nuclear and mitochondrial genomes of the same species. Biases in single nucleotide and dinucleotide representation were found to be the major determinants of global structure in mitochondrial and nuclear genomes, respectively. Analysis of DNA sequence evolution must therefore incorporate both gene and genome-type specific mutational and selective constraints.
- OSTI ID:
- 133902
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-0636
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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