MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates

Journal Article · Thu Nov 24 00:00:00 EST 2016 · Analytical Biochemistry

DOI:https://doi.org/10.1016/j.ab.2016.11.014· OSTI ID:1335584

Van Wychen, Stefanie ^[1]; Long, William ^[1]; Black, Stuart K. ^[1]; Laurens, Lieve M. L. ^[1]

---

1. National Renewable Energy Lab. (NREL), Golden, CO (United States)

A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. As a result, the MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 ug mL^-1 without interference from other algae acidic hydrolyzate components.

• Research Organization: NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))
• Sponsoring Organization: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
• Grant/Contract Number: AC36-08GO28308
• OSTI ID: 1335584
• Alternate ID(s): OSTI ID: 1396852
• Report Number(s): NREL/JA--5100-67563
• Journal Information: Analytical Biochemistry, Journal Name: Analytical Biochemistry Journal Issue: C Vol. 518; ISSN 0003-2697
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
MBTH
algae
carbohydrates
phenol-sulfuric acid
spectrophotometric