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Determination of Total Carbohydrates in Algal Biomass: Laboratory Analytical Procedure (LAP)

Technical Report ·
DOI:https://doi.org/10.2172/2208753· OSTI ID:2208753
This Laboratory Analytical Procedure (LAP) uses a two-step sulfuric acid hydrolysis to hydrolyze polymeric forms of carbohydrates in algal biomass into monomeric subunits. The monomers are then quantified by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) or a suitable spectrophotometric method.
Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
DOE Contract Number:
AC36-08GO28308
OSTI ID:
2208753
Report Number(s):
NREL/TP--2700-87500; MainId:88275; UUID:76c40f90-922e-43b4-a669-3fb59e875485; MainAdminID:71001
Country of Publication:
United States
Language:
English