Genome-wide analysis on Chlamydomonas reinhardtii reveals the impact of hydrogen peroxide on protein stress responses and overlap with other stress transcriptomes

Blaby, Ian K.; Blaby-Haas, Crysten E.; Pérez-Pérez, María Esther; Schmollinger, Stefan; Fitz-Gibbon, Sorel; Lemaire, Stéphane D.; Merchant, Sabeeha S.

doi:10.1111/tpj.13053

Title: Genome-wide analysis on Chlamydomonas reinhardtii reveals the impact of hydrogen peroxide on protein stress responses and overlap with other stress transcriptomes

Journal Article · Mon Dec 07 00:00:00 EST 2015 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.13053· OSTI ID:1335416

Blaby, Ian K. ^[1]; Blaby-Haas, Crysten E. ^[1]; Pérez-Pérez, María Esther ^[2]; Schmollinger, Stefan ^[3]; Fitz-Gibbon, Sorel ^[4]; Lemaire, Stéphane D. ^[5]; Merchant, Sabeeha S. ^[6]

Univ. of California, Los Angeles, CA (United States). Department of Chemistry and Biochemistry; Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Department
Sorbonne Universites, UPMC Univ., Paris (France); Laboratoire de Biologie Moleculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Paris (France); Instituto de BioquiÂmica Vegetal y FotosiÂntesis, Consejo Superior de Investigaciones CientiÂficas (CSIC)-Universidad de Sevilla, Seville (Spain)
Univ. of California, Los Angeles, CA (United States). Department of Chemistry and Biochemistry
Univ. of California, Los Angeles, CA (United States). Institute for Genomics and Proteomics
Sorbonne Universites, UPMC Univ., Paris (France); Laboratoire de Biologie Moleculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Paris (France)
Univ. of California, Los Angeles, CA (United States). Department of Chemistry and Biochemistry; Univ. of California, Los Angeles, CA (United States). Institute for Genomics and Proteomics

Reactive oxygen species (ROS) are produced by and have the potential to be damaging to all aerobic organisms. In photosynthetic organisms, they are an unavoidable byproduct of electron transfer in both the chloroplast and mitochondrion. Here, in this paper, we employ the reference unicellular green alga Chlamydomonas reinhardtii to identify the effect of H₂O₂ on gene expression by monitoring the changes in the transcriptome in a time-course experiment. Comparison of transcriptomes from cells sampled immediately prior to the addition of H₂O₂ and 0.5 and 1 h subsequently revealed 1278 differentially abundant transcripts. Of those transcripts that increase in abundance, many encode proteins involved in ROS detoxification, protein degradation and stress responses, whereas among those that decrease are transcripts encoding proteins involved in photosynthesis and central carbon metabolism. In addition to these transcriptomic adjustments, we observe that addition of H₂O₂ is followed by an accumulation and oxidation of the total intracellular glutathione pool, and a decrease in photosynthetic O₂ output. Additionally, we analyze our transcriptomes in the context of changes in transcript abundance in response to singlet O₂ (O₂^*), and relate our H₂O₂-induced transcripts to a diurnal transcriptome, where we demonstrate enrichments of H₂O₂-induced transcripts early in the light phase, late in the light phase and 2 h prior to light. In conclusion, on this basis several genes that are highlighted in this work may be involved in previously undiscovered stress remediation pathways or acclimation responses.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012704; FD02-04ER15529

OSTI ID:: 1335416

Report Number(s):: BNL-111999-2016-JA

Journal Information:: The Plant Journal, Vol. 84, Issue 5; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 34 works

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