Hydrogen-peroxide-induced oxidative stress responses in Desulfovibrio vulgaris Hildenborough

Journal Article · Thu Jul 01 04:00:00 EDT 2010 · Environmental Microbiology

DOI:https://doi.org/10.1111/j.1462-2920.2010.02234.x· OSTI ID:993496

Zhou, A; He, Z; Redding-Johanson, A M; Mukhopadhyay, A; Hemme, C L; Joachimiak, M P; Bender, K S; Keasling, J D; Stahl, D A; Fields, M W; Hazen, T C; Arkin, A P; Wall, J D; Zhou, J; Luo, F; Deng, Y; He, Q

To understand how sulphate-reducing bacteria respond to oxidative stresses, the responses of Desulfovibrio vulgaris Hildenborough to H{sub 2}O{sub 2}-induced stresses were investigated with transcriptomic, proteomic and genetic approaches. H{sub 2}O{sub 2} and induced chemical species (e.g. polysulfide, ROS) and redox potential shift increased the expressions of the genes involved in detoxification, thioredoxin-dependent reduction system, protein and DNA repair, and decreased those involved in sulfate reduction, lactate oxidation and protein synthesis. A gene coexpression network analysis revealed complicated network interactions among differentially expressed genes, and suggested possible importance of several hypothetical genes in H{sub 2}O{sub 2} stress. Also, most of the genes in PerR and Fur regulons were highly induced, and the abundance of a Fur regulon protein increased. Mutant analysis suggested that PerR and Fur are functionally overlapped in response to stresses induced by H{sub 2}O{sub 2} and reaction products, and the upregulation of thioredoxin-dependent reduction genes was independent of PerR or Fur. It appears that induction of those stress response genes could contribute to the increased resistance of deletion mutants to H{sub 2}O{sub 2}-induced stresses. In addition, a conceptual cellular model of D. vulgaris responses to H{sub 2}O{sub 2} stress was constructed to illustrate that this bacterium may employ a complicated molecular mechanism to defend against the H{sub 2}O{sub 2}-induced stresses.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
• Sponsoring Organization: Earth Sciences Division
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 993496
• Report Number(s): LBNL-4009E
• Journal Information: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 10 Vol. 12; ISSN 1462-2912
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
58 GEOSCIENCES
ABUNDANCE
BACTERIA
DESULFOVIBRIO
DETOXIFICATION
DNA REPAIR
GENES
GENETICS
HYDROGEN PEROXIDE
INDUCTION
LACTATES
MUTANTS
NETWORK ANALYSIS
OXIDATION
PROTEINS
REDOX POTENTIAL
STRESSES
SULFATE-REDUCING BACTERIA
SULFATES
SYNTHESIS