Cloning of a tumor suppressor gene (TSG) on chromosome 3p13 involved in a subset of breast cancer

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · American Journal of Human Genetics

OSTI ID:133500

Geil, L ^[1]; Latif, F; Lerman, M I ^[2]

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1. Program Resources, Inc./DynCorp, Frederick, MD (United States)
2. NCI-FCRDC, Frederick, MD (United States); and others

There is evidence for a TSG involved in breast cancer on the short arm of chromosome 3. This has been shown by loss of heterozygosity (LOH) data. The area is divided into two distinct regions, one at 3p24-26 (LOH 45%) and the other at 3p13-14 (LOH 41%). While looking for LOH a homozygous deletion was found in one breast tumor with a cosmid probe located at 3p13 using fluorescence in situ hybridization (FISH) analysis. A Yac of 1.5 Mb was isolated and characterized. The Yac is stable and is not chimeric. The left and the right ends of the Yac were converted into large phages -20 Kb and FISH analysis is being used to determine if either or both ends of the Yac are still in the deletion or are outside of the deletion. The Yac was converted into cosmids and the cosmids are being used to isolate candidate genes in the region 3p13. We have also shown that the 3p13 breast homozygous deletion does not overlap with the U2020 small cell lung homozygous deletion at 3p13-12.

• OSTI ID: 133500
• Report Number(s): CONF-941009--
• Journal Information: American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMAL ABERRATIONS
DNA HYBRIDIZATION
DNA-CLONING
FLUORESCENCE
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 13
MAMMARY GLANDS
NEOPLASMS
PROBES
STATISTICS
YEASTS