Molecular cloning and characterization of 3q21 breakpoints from leukemia-associated t(3;3)(q21;q26)
Journal Article
·
· American Journal of Human Genetics
OSTI ID:133335
- Eleanor Roosevelt Institute, Denver, CO (United States)
- Karolinska Institute, Stockholm (Sweden); and others
A variety of rearrangements of 3q21, including deletion, translocation with 3q26 and other chromosomes, and inversion of 3q21-3q26, have been associated with hematological malignancy. To begin characterization of the 3q21 breakpoint region, the der3q- and der3q+ chromosomes from 2 separate leukemia patients carrying t(3;3)(q21;q26) have been isolated in hybrid cell lines, and the der3q- chromosomes analyzed extensively. In both cases, the 3q26 breakpoints occur between the EAP and EVI1 genes without direct rearrangement of either gene. In 3q21, 2 NotI linking clones have been identified that are physically linked on a 50 kb NotI fragment that spans both 3q21 breakpoints. To characterize this region further, an 80 kb P1 clone containing both NotI clones has been isolated from the Dupont total human P1 library. The breakpoints have been localized within this clone to SacI fragments of 1 kb and 0.8 kb that are separated by 9 kb. This is preliminary evidence suggesting that the 3q21 leukemia breakpoints may be generally more clustered than those within 3q26. Rare restriction site mapping of the P1 clone shows a high density of sites and suggests a GC-rich and therefore potentially gene-rich region. Sequence analysis of the breakpoint fragments has so far yielded no homologies with known genes; however, preliminary analysis around the NotI site 35 kb centromeric to the breakpoints shows homology with several transcription factor genes. Isolation of all genes within the P1 clone is in progress.
- OSTI ID:
- 133335
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
Similar Records
Consistent intergenic splicing and production of multiple transcripts between AML1 at 21q22 and unrelated genes at 3q26 in (3;21)(q26;q22) translocations
Chromosome walking on the TCL1 locus involved in T-cell neoplasia
Mapping chromosome band 11q23 in human acute leukemia with biotinylated probes: Identification of 11q23 translocation breakpoints with a yeast artificial chromosome
Journal Article
·
Tue Apr 26 00:00:00 EDT 1994
· Proceedings of the National Academy of Sciences of the United States of America
·
OSTI ID:86510
Chromosome walking on the TCL1 locus involved in T-cell neoplasia
Journal Article
·
Fri Oct 15 00:00:00 EDT 1993
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5147423
Mapping chromosome band 11q23 in human acute leukemia with biotinylated probes: Identification of 11q23 translocation breakpoints with a yeast artificial chromosome
Journal Article
·
Fri Nov 30 23:00:00 EST 1990
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:6203112