A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni

Day, Christopher J.; King, Rebecca M.; Shewell, Lucy K.; Tram, Greg; Najnin, Tahria; Hartley-Tassell, Lauren E.; Wilson, Jennifer C.; Fleetwood, Aaron D.; Zhulin, Igor B.; Korolik, Victoria

doi:10.1038/ncomms13206

A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni

Journal Article · Thu Oct 20 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms13206· OSTI ID:1330560

Day, Christopher J. ^[1]; King, Rebecca M. ^[1]; Shewell, Lucy K. ^[1]; Tram, Greg ^[1]; Najnin, Tahria ^[1]; Hartley-Tassell, Lauren E. ^[1]; Wilson, Jennifer C. ^[1]; Fleetwood, Aaron D. ^[2]; ^[3]; Korolik, Victoria ^[1]

Griffith Univ., Queensland (Australia)
Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

A rare chemotaxis receptor, Tlp11, has been previously identified in invasive strains of Campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. Here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that Tlp11 specifically interacts with galactose. Tlp11 is required for the chemotactic response of C. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. The inactivated mutant displays reduced virulence in vivo, in a model of chicken colonization. The Tlp11 sensory domain represents the first known sugar-binding dCache_1 domain, which is the most abundant family of extracellular sensors in bacteria. The Tlp11 signalling domain interacts with the chemotaxis scaffolding proteins CheV and CheW, and comparative genomic analysis indicates a likely recent evolutionary origin for Tlp11. Lastly, we propose to rename Tlp11 as CcrG, Campylobacter ChemoReceptor for Galactose.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1330560

Journal Information:: Nature Communications, Journal Name: Nature Communications Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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