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Title: Orenia metallireducens sp. nov. strain Z6, a Novel Metal-reducing [member of the Phylum] Firmicute from the Deep Subsurface

Journal Article · · Applied and Environmental Microbiology
DOI:https://doi.org/10.1128/AEM.02382-16· OSTI ID:1330336
 [1];  [1];  [2];  [2];  [2];  [2];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of Illinois, Urbana-Champaign, Urbana, IL (United States)
  2. Argonne National Lab. (ANL), Lemont, IL (United States)
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A novel halophilic and metal-reducing bacterium, Orenia metallireducens strain Z6, was isolated from briny groundwater extracted from a 2.02 km-deep borehole in the Illinois Basin, IL. This organism shared 96% 16S rRNA gene similarity with Orenia marismortui but demonstrated physiological properties previously unknown for this genus. In addition to exhibiting a fermentative metabolism typical of the genus Orenia, strain Z6 reduces various metal oxides [Fe(III), Mn(IV), Co(III), and Cr(VI)], using H2 as the electron donor. Strain Z6 actively reduced ferrihydrite over broad ranges of pH (6 to 9.6), salinity (0.4 to 3.5 M NaCl), and temperature (20 to 60°C). At pH 6.5, strain Z6 also reduced more crystalline iron oxides, such as lepidocrocite (γ-FeOOH), goethite (α-FeOOH), and hematite (α-Fe2O3). Analysis of X-ray absorption fine structure (XAFS) following Fe(III) reduction by strain Z6 revealed spectra from ferrous secondary mineral phases consistent with the precipitation of vivianite [Fe3(PO4)2] and siderite (FeCO3). The draft genome assembled for strain Z6 is 3.47 Mb in size and contains 3,269 protein-coding genes. Unlike the well-understood iron-reducing Shewanella and Geobacter species, this organism lacks the c-type cytochromes for typical Fe(III) reduction. Strain Z6 represents the first bacterial species in the genus Orenia (order Halanaerobiales) reported to reduce ferric iron minerals and other metal oxides. This microbe expands both the phylogenetic and physiological scopes of iron-reducing microorganisms known to inhabit the deep subsurface and suggests new mechanisms for microbial iron reduction. In conclusion, these distinctions from other Orenia spp. support the designation of strain Z6 as a new species, Orenia metallireducens sp. nov.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23), Subsurface Biogeochemical Research; National Aeronautics and Space Administration (NASA)
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1330336
Journal Information:
Applied and Environmental Microbiology, Vol. 82, Issue 21; ISSN 0099-2240
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 21 works
Citation information provided by
Web of Science

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Cited By (4)

Impact of iron reduction on the metabolism of Clostridium acetobutylicum journal May 2019
Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32 journal July 2019
A flavin-based extracellular electron transfer mechanism in diverse Gram-positive bacteria journal September 2018
A flavin-based extracellular electron transfer mechanism in diverse Gram-positive bacteria. text January 2018

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