Field Evaluation of Transgenic Switchgrass Plants Overexpressing PvMYB4 for Reduced Biomass Recalcitrance

Baxter, Holly L.; Poovaiah, Charleson R.; Yee, Kelsey L.; Mazarei, Mitra; Rodriguez, Miguel; Thompson, Olivia A.; Shen, Hui; Turner, Geoffrey B.; Decker, Stephen R.; Sykes, Robert W.; Chen, Fang; Davis, Mark F.; Mielenz, Jonathan R.; Davison, Brian H.; Dixon, Richard A.; Stewart, C. Neal

doi:10.1007/s12155-014-9570-1

Title: Field Evaluation of Transgenic Switchgrass Plants Overexpressing PvMYB₄ for Reduced Biomass Recalcitrance

Journal Article · Wed Jan 07 00:00:00 EST 2015 · BioEnergy Research

DOI:https://doi.org/10.1007/s12155-014-9570-1· OSTI ID:1327634

Baxter, Holly L. ^[1]; Poovaiah, Charleson R. ^[1]; Yee, Kelsey L. ^[2]; Mazarei, Mitra ^[1]; Rodriguez, Miguel ^[2]; Thompson, Olivia A. ^[2]; Shen, Hui ^[3]; Turner, Geoffrey B. ^[4]; Decker, Stephen R. ^[4]; Sykes, Robert W. ^[4]; Chen, Fang ^[3]; Davis, Mark F. ^[4]; Mielenz, Jonathan R. ^[2]; Davison, Brian H. ^[2]; Dixon, Richard A. ^[3]; Stewart, C. Neal ^[1]

Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC) and Biosciences Divison
Univ. of North Texas, Denton, TX (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)

High biomass yields and minimal agronomic input requirements have made switchgrass, Panicum virgatum L., a leading candidate lignocellulosic bioenergy crop. Large-scale lignocellulosic biofuel production from such crops is limited by the difficulty to deconstruct cell walls into fermentable sugars: the recalcitrance problem. In this study, we assessed the field performance of switchgrass plants overexpressing the switchgrass MYB₄ (PvMYB₄) transcription factor gene. PvMYB₄ transgenic switchgrass can have great lignin reduction, which commensurately increases sugar release and biofuel production. Our results over two growing seasons showed that one transgenic event (out of eight) had important gains in both biofuel (32% more) and biomass (63% more) at the end of the second growing season relative to non-transgenic controls. These gains represent a doubling of biofuel production per hectare, which is the highest gain reported from any field-grown modified feedstock. In contrast to this transgenic event, which had relatively low ectopic overexpression of the transgene, five of the eight transgenic events planted did not survive the first field winter. The dead plants were all high-overexpressing events that performed well in the earlier greenhouse studies. Disease susceptibility was not compromised in any transgenic events over the field experiments. These results demonstrate the power of modifying the expression of an endogenous transcription factor to improve biofuel and biomass simultaneously, and also highlight the importance of field studies for "sorting" transgenic events. In conclusion, further research is needed to develop strategies for fine-tuning temporal-spatial transgene expression in feedstocks to optimize desired phenotypes.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1327634

Journal Information:: BioEnergy Research, Vol. 8, Issue 3; ISSN 1939-1234

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 39 works

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Title: Field Evaluation of Transgenic Switchgrass Plants Overexpressing PvMYB4 for Reduced Biomass Recalcitrance

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