Identification and overexpression of a knotted1-like transcription factor in switchgrass (Panicum virgatum L.) for lignocellulosic feedstock improvement

Wuddineh, Wegi A.; Mazarei, Mitra; Zhang, Ji -Yi; Turner, Geoffrey B.; Sykes, Robert W.; Decker, Stephen R.; Davis, Mark F.; Udvardi, Michael K.

doi:10.3389/fpls.2016.00520

Identification and overexpression of a knotted1-like transcription factor in switchgrass (Panicum virgatum L.) for lignocellulosic feedstock improvement

Journal Article · Thu Apr 28 00:00:00 EDT 2016 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2016.00520· OSTI ID:1254112

Wuddineh, Wegi A. ^[1]; Mazarei, Mitra ^[1]; Zhang, Ji -Yi ^[2]; Turner, Geoffrey B. ^[3]; Sykes, Robert W. ^[3]; Decker, Stephen R. ^[3]; Davis, Mark F. ^[3]; Udvardi, Michael K. ^[2]

Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Samuel Roberts Noble Foundation, Ardmore, OK (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)

High biomass production and wide adaptation has made switchgrass (Panicum virgatum L.) an important candidate lignocellulosic bioenergy crop. One major limitation of this and other lignocellulosic feedstocks is the recalcitrance of complex carbohydrates to hydrolysis for conversion to biofuels. Lignin is the major contributor to recalcitrance as it limits the accessibility of cell wall carbohydrates to enzymatic breakdown into fermentable sugars. Therefore, genetic manipulation of the lignin biosynthesis pathway is one strategy to reduce recalcitrance. Here, we identified a switchgrass Knotted1 transcription factor, PvKN1, with the aim of genetically engineering switchgrass for reduced biomass recalcitrance for biofuel production. Gene expression of the endogenous PvKN1 gene was observed to be highest in young inflorescences and stems. Ectopic overexpression of PvKN1 in switchgrass altered growth, especially in early developmental stages. Transgenic lines had reduced expression of most lignin biosynthetic genes accompanied by a reduction in lignin content suggesting the involvement of PvKN1 in the broad regulation of the lignin biosynthesis pathway. Moreover, the reduced expression of the Gibberellin 20-oxidase (GA20ox) gene in tandem with the increased expression of Gibberellin 2-oxidase (GA2ox) genes in transgenic PvKN1 lines suggest that PvKN1 may exert regulatory effects via modulation of GA signaling. Furthermore, overexpression of PvKN1 altered the expression of cellulose and hemicellulose biosynthetic genes and increased sugar release efficiency in transgenic lines. Our findings demonstrated that switchgrass PvKN1 is a putative ortholog of maize KN1 that is linked to plant lignification and cell wall and development traits as a major regulatory gene. Therefore, targeted overexpression of PvKN1 in bioenergy feedstocks may provide one feasible strategy for reducing biomass recalcitrance and simultaneously improving plant growth characteristics.

View Accepted Manuscript (DOE)

Research Organization:: NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1254112

Report Number(s):: NREL/JA--2700-66232

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Journal Issue: 7 Vol. 7; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Suberin and hemicellulose in sugarcane cell wall architecture and crop digestibility: A biotechnological perspective Figueiredo, Raquel; Araújo, Pedro; Llerena, Juan Pablo P. Food and Energy Security, Vol. 8, Issue 3 https://doi.org/10.1002/fes3.163	journal	February 2019
Genetic engineering of grass cell wall polysaccharides for biorefining Bhatia, Rakesh; Gallagher, Joe A.; Gomez, Leonardo D. Plant Biotechnology Journal, Vol. 15, Issue 9 https://doi.org/10.1111/pbi.12764	journal	June 2017
Development and use of a switchgrass (Panicum virgatum L.) transformation pipeline by the BioEnergy Science Center to evaluate plants for reduced cell wall recalcitrance Nelson, Richard S.; Stewart, C. Neal; Gou, Jiqing Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0991-x	journal	December 2017
Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass Liu, Wusheng; Mazarei, Mitra; Ye, Rongjian Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1119-7	journal	April 2018

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