Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice

Yin, Yufeng; Zhao, Lidan; Zhang, Fengchun; Zhang, Xuan

doi:10.1038/srep31874

Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice

Journal Article · Mon Aug 22 00:00:00 EDT 2016 · Scientific Reports

DOI:https://doi.org/10.1038/srep31874· OSTI ID:1326665

Yin, Yufeng ^[1]; Zhao, Lidan ^[2]; Zhang, Fengchun ^[2]; Zhang, Xuan ^[2]

Peking Union Medical College Hospital, Beijing (China). Key Lab. of Rheumatology & Clinical Immunology, Dept. of Rheumatology & Clinical Immunology; Chinese Academy of Medical Science (CAS), Beijing (China). Clinical Immunology Center; none
Peking Union Medical College Hospital, Beijing (China). Key Lab. of Rheumatology & Clinical Immunology, Dept. of Rheumatology & Clinical Immunology; Chinese Academy of Medical Science (CAS), Beijing (China). Clinical Immunology Center

Abnormal expression of CD200/CD200R1 may contribute to the immunologic abnormalities in patients with systemic lupus erythematosus (SLE). This study aimed to assess the function of CD200/CD200R1and impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice. Female NZB/WF1 mice were treated with CD200-Fc or control for 4 weeks. Plasma samples were collected to measure autoantibody levels. The expression levels of CD200/CD200R1 in peripheral blood mononuclear cells (PBMCs) and splenocytes were examined. The percentage of CD200/CD200R1-positive cells in splenocytes from NZB/WF1 mice was lower than that of C57BL/6 mice (p<0.05). The plasma level of anti-dsDNA was significantly higher in NZB/WF1 mice than C57BL/6 mice (p<0.001). However, the anti-dsDNA levels decreased (p=0.047) after CD200-Fc treatment. Finally, CD200-Fc reduced the levels of IL-6 (p=0.017) and IL-10 (p=0.03) in the dendritic cell culture supernatant. This study suggests that the immunosuppressive CD200/CD200R1 signaling pathway might be involved in the immunopathology of NZB/WF1 mice; the present results merit further exploration of agents that can modulate the CD200/CD200FR1 pathway as a therapy for human lupus.

View Accepted Manuscript (DOE)

Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: Beijing Municipal Natural Science Foundation; National Natural Science Foundation of China; USDOE

Grant/Contract Number:: SC0001035

OSTI ID:: 1326665

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 6; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
CD200-Fc
Lupus
Lupus nephritis
NZB/WF1

Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice

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References (26)

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