Induction of a systemic lupus erythematosus-like disease in mice by a common human anti-DNA idiotype

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.7.2260· OSTI ID:7009395

Mendlovic, S; Brocke, S; Meshorer, A; Mozes, E ^[1]; Shoenfeld, Y; Bakimer, R ^[2]; Ben-Bassat, M ^[3]

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1. Weizmann Institute of Science, Rehovot (Israel)
2. Ben-Gurion Univ., Beer-Sheva (Israel)
3. Beilinson Medical Center, Petah-Tiqva (Israel)

Systemic lupus erythematosus (SLE) is considered to be the quintessential autoimmune disease. It has not been possible to induce SLE in animal models by DNA immunization or by challenge with anti-DNA antibodies. The authors report a murine model of SLE-like disease induced by immunization of C3H.SW female mice with a common human monoclonal anti-DNA idiotype (16/6 idiotype). Following a booster injection with the 16/6 idiotype, high levels of murine anti-16/6 and anti-anti-16/6 antibodies (associated with anti-DNA activity) were detected in the sera of the immunized mice. Elevated titers of autoantibodies reacting with DNA, poly(I), poly(dT), ribonucleoprotein, autoantigens (Sm, SS-A (Ro), and SS-B (La)), and cardiolipin were noted. The serological findings were associated with increased erythrocyte sedimentation rate, leukopenia, proteinuria, immune complex deposition in the glomerular mesangium, and sclerosis of the glomeruli. The immune complexes in the kidneys were shown to contain the 16/6 idiotype. This experimental SLE-like model may be used to elucidate the mechanisms underlying SLE.

• OSTI ID: 7009395
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:7; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
ANTIGENS
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHEMISTRY
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ENZYME IMMUNOASSAY
ERYTHROCYTES
GLOBULINS
HEMIC DISEASES
HISTOLOGY
IMMUNE SYSTEM DISEASES
IMMUNITY
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LEUKOPENIA
LUPUS
MAMMALS
MATERIALS
MICE
MICROSCOPY
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SKIN DISEASES
TRACER TECHNIQUES
TRANSMISSION ELECTRON MICROSCOPY
TUBERCULOSIS
VERTEBRATES