Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants

Li, Shundai; Bashline, Logan; Zheng, Yunzhen; Xin, Xiaoran; Huang, Shixin; Kong, Zhaosheng; Kim, Seong H.; Cosgrove, Daniel J.; Gu, Ying

doi:10.1073/pnas.1613273113

Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants

Journal Article · Mon Sep 19 00:00:00 EDT 2016 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1613273113· OSTI ID:1325833

Li, Shundai ^[1]; Bashline, Logan ^[1]; Zheng, Yunzhen ^[2]; Xin, Xiaoran ^[1]; Huang, Shixin ^[3]; Kong, Zhaosheng ^[4]; Kim, Seong H. ^[3]; Cosgrove, Daniel J. ^[2]; Gu, Ying ^[1]

Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802,
Department of Biology, Pennsylvania State University, University Park, PA 16802,
Chemical Engineering and Materials Research Institute, Pennsylvania State University, University Park, PA 16802,
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Significance

Plant cell walls are important in plant development and for textiles, wood products, and bioenergy. Cellulose, the microfibrillar component of primary cell walls (PCWs) and secondary cell walls (SCWs), is formed by cellulose synthase complexes (CSCs) at the plasma membrane. Here, we show that CSCs behave differently during PCW and SCW synthesis and form microfibrils with different organization. During PCW synthesis, dispersed CSCs synthesize cellulose microfibrils with low aggregation, whereas during SCW synthesis, densely arranged groups of CSCs move coherently to synthesize highly aggregated microfibrils. Our study suggests that controlled alterations in CSC distribution and orchestrated movements contribute to the high density and bundling of cellulose microfibrils in SCWs.

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Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF)

Sponsoring Organization:: USDOE; USDOE SC Office of Basic Energy Sciences (SC-22)

Grant/Contract Number:: SC0001090

OSTI ID:: 1325833

Alternate ID(s):: OSTI ID: 1388836

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 40 Vol. 113; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants

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