A single heterologously expressed plant cellulose synthase isoform is sufficient for cellulose microfibril formation in vitro

Purushotham, Pallinti; Cho, Sung Hyun; Díaz-Moreno, Sara M.; Kumar, Manish; Nixon, B. Tracy; Bulone, Vincent; Zimmer, Jochen

doi:10.1073/pnas.1606210113

A single heterologously expressed plant cellulose synthase isoform is sufficient for cellulose microfibril formation in vitro

Journal Article · Mon Sep 19 04:00:00 EDT 2016 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1606210113· OSTI ID:1325834

Purushotham, Pallinti ^[1]; Cho, Sung Hyun ^[2]; Díaz-Moreno, Sara M. ^[3]; Kumar, Manish ^[4]; Nixon, B. Tracy ^[2]; Bulone, Vincent ^[5]; Zimmer, Jochen ^[1]

Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908,
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802,
Division of Glycoscience, Royal Institute of Technology, Stockholm, SE-10691, Sweden,
Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802,
Division of Glycoscience, Royal Institute of Technology, Stockholm, SE-10691, Sweden,, Australian Research Council Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia

Significance

Cellulose is an abundant natural polymer synthesized primarily by vascular plants in which it forms the load-bearing component of the cell wall. It is a linear polymer of glucose molecules synthesized by membrane-embedded cellulose synthases that couple polymer synthesis with its secretion across the plasma membrane. Plants express multiple cellulose synthase isoforms that are organized into large macromolecular assemblies of varying composition that are likely responsible for aligning the cellulose strands into microfibrils. Here we show that recombinantly expressed and purified Populus tremula x tremuloides (hybrid aspen) cellulose synthase-8 is sufficient for cellulose biosynthesis and produces cellulose microfibrils in vitro. Our results demonstrate that no other plant-derived factors are required for cellulose microfibril biosynthesis.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0001090

OSTI ID:: 1325834

Alternate ID(s):: OSTI ID: 1388837

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 40 Vol. 113; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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