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1,2-Propanediol Dehydration in Roseburia inulinivorans: Structural Basis for Substrate and Enantiomer Selectivity

Journal Article · · Journal of Biological Chemistry
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  1. Univ. of Georgia, Athens, GA (United States)
  2. Braskem S.A., São Paulo, SP (Brazil)
+ Show Author Affiliations
Glycyl radical enzymes (GREs) represent a diverse superfamily of enzymes that utilize a radical mechanism to catalyze difficult, but often essential, chemical reactions. Here, we present the first biochemical and structural data for a GRE-type diol dehydratase from the organism Roseburia inulinivorans (RiDD). Despite high sequence (48% identity) and structural similarity to the GRE-type glycerol dehydratase from Clostridium butyricum, we demonstrate that the RiDD is in fact a diol dehydratase. In addition, the RiDD will utilize both (S)-1,2- propanediol and (R)-1,2-propanediol as a substrate, with an observed preference for the S enantiomer. Based on the new structural information we developed and successfully tested a hypothesis that explains the functional differences we observe.
Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
USDOE
OSTI ID:
1324768
Journal Information:
Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 30 Vol. 291; ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular BiologyCopyright Statement
Country of Publication:
United States
Language:
ENGLISH

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Cited By (7)

Bacterial microcompartments journal March 2018
Metabolic functions of the human gut microbiota: the role of metalloenzymes journal January 2019
A glycyl radical enzyme enables hydrogen sulfide production by the human intestinal bacterium Bilophila wadsworthia journal February 2019
New tricks for the glycyl radical enzyme family journal September 2017
A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans -4-hydroxy-l-proline journal February 2017
Glycyl Radical Enzyme-Associated Microcompartments: Redox-Replete Bacterial Organelles journal January 2019
Bacterial Microcompartments journal October 2010

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
chemical biology
diol dehydratase
enzyme structure
glycerol dehydratase
glycyl radical enzyme
intestinal metabolism
metabolic engineering
radical
radical dehydration