Large-Scale Metabolite Analysis of Standards and Human Serum by Laser Desorption Ionization Mass Spectrometry from Silicon Nanopost Arrays

Korte, Andrew R.; Stopka, Sylwia A.; Morris, Nicholas; Razunguzwa, Trust; Vertes, Akos

doi:10.1021/acs.analchem.6b01186

Title: Large-Scale Metabolite Analysis of Standards and Human Serum by Laser Desorption Ionization Mass Spectrometry from Silicon Nanopost Arrays

Journal Article · Fri Jul 22 00:00:00 EDT 2016 · Analytical Chemistry

DOI:https://doi.org/10.1021/acs.analchem.6b01186· OSTI ID:1315834

Korte, Andrew R. ^[1]; Stopka, Sylwia A. ^[1]; Morris, Nicholas ^[2]; Razunguzwa, Trust ^[2]; Vertes, Akos ^[1]

Department of Chemistry, George Washington University, 800 22nd Street, North West, Washington, District of Columbia 20052, United States
Protea Biosciences, Inc., Morgantown, West Virginia 26505, United States

The unique challenges presented by metabolomics have driven the development of new mass spectrometry (MS)-based techniques for small molecule analysis. We have previously demonstrated silicon nanopost arrays (NAPA) to be an effective substrate for laser desorption ionization (LDI) of small molecules for MS. However, the utility of NAPA-LDI-MS for a wide range of metabolite classes has not been investigated. Here we apply NAPA-LDI-MS to the large-scale acquisition of high-resolution mass spectra and tandem mass spectra from a collection of metabolite standards covering a range of compound classes including amino acids, nucleotides, carbohydrates, xenobiotics, lipids, and other classes. In untargeted analysis of metabolite standard mixtures, detection was achieved for 374 compounds and useful MS/MS spectra were obtained for 287 compounds, without individual optimization of ionization or fragmentation conditions. Metabolite detection was evaluated in the context of 31 metabolic pathways, and NAPA-LDI-MS was found to provide detection for 63% of investigated pathway metabolites. Individual, targeted analysis of the 20 common amino acids provided detection of 100% of the investigated compounds, demonstrating that improved coverage is possible through optimization and targeting of individual analytes or analyte classes. In direct analysis of aqueous and organic extracts from human serum samples, spectral features were assigned to a total of 108 small metabolites and lipids. Glucose and amino acids were quantitated within their physiological concentration ranges. Finally, the broad coverage demonstrated by this large-scale screening experiment opens the door for use of NAPA-LDI-MS in numerous metabolite analysis applications

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Research Organization:: George Washington Univ., Washington, DC (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-01ER15129; W911NF-14-2-0020

OSTI ID:: 1315834

Alternate ID(s):: OSTI ID: 1326648

Journal Information:: Analytical Chemistry, Journal Name: Analytical Chemistry Vol. 88 Journal Issue: 18; ISSN 0003-2700

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 32 works

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Matrix‐free mass spectrometry imaging of mouse brain tissue sections on silicon nanopost arrays Fincher, Jarod A.; Dyer, Jacqueline E.; Korte, Andrew R. Journal of Comparative Neurology, Vol. 527, Issue 13 https://doi.org/10.1002/cne.24566	journal	December 2018
Mass spectrometry imaging of triglycerides in biological tissues by laser desorption ionization from silicon nanopost arrays Fincher, Jarod A.; Korte, Andrew R.; Dyer, Jacqueline E. Journal of Mass Spectrometry, Vol. 55, Issue 4 https://doi.org/10.1002/jms.4443	journal	December 2019
Construction of a Plasmonic Chip for Metabolic Analysis in Cervical Cancer Screening and Evaluation Shu, Weikang; Wang, You; Liu, Chao Small Methods, Vol. 4, Issue 4 https://doi.org/10.1002/smtd.201900469	journal	April 2020
MALDI matrices for low molecular weight compounds: an endless story? Calvano, Cosima Damiana; Monopoli, Antonio; Cataldi, Tommaso R. I. Analytical and Bioanalytical Chemistry, Vol. 410, Issue 17 https://doi.org/10.1007/s00216-018-1014-x	journal	April 2018
Atmospheric Pressure Plasma-Treated Carbon Nanowalls’ Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (CNW-SALDI-MS) Ohta, Takayuki; Ito, Hironori; Ishikawa, Kenji C, Vol. 5, Issue 3 https://doi.org/10.3390/c5030040	journal	July 2019

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