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Title: Modified host cells with efflux pumps

Abstract

The present invention provides for a modified host cell comprising a heterologous expression of an efflux pump capable of transporting an organic molecule out of the host cell wherein the organic molecule at a sufficiently high concentration reduces the growth rate of or is lethal to the host cell.

Inventors:
; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1312679
Patent Number(s):
9,428,726
Application Number:
13/115,925
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 May 25
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Dunlop, Mary J., Keasling, Jay D., and Mukhopadhyay, Aindrila. Modified host cells with efflux pumps. United States: N. p., 2016. Web.
Dunlop, Mary J., Keasling, Jay D., & Mukhopadhyay, Aindrila. Modified host cells with efflux pumps. United States.
Dunlop, Mary J., Keasling, Jay D., and Mukhopadhyay, Aindrila. Tue . "Modified host cells with efflux pumps". United States. https://www.osti.gov/servlets/purl/1312679.
@article{osti_1312679,
title = {Modified host cells with efflux pumps},
author = {Dunlop, Mary J. and Keasling, Jay D. and Mukhopadhyay, Aindrila},
abstractNote = {The present invention provides for a modified host cell comprising a heterologous expression of an efflux pump capable of transporting an organic molecule out of the host cell wherein the organic molecule at a sufficiently high concentration reduces the growth rate of or is lethal to the host cell.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {8}
}

Patent:

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Works referenced in this record:

Antibiotic-Dependent Induction of Pseudomonas putida DOT-T1E TtgABC Efflux Pump Is Mediated by the Drug Binding Repressor TtgR
journal, September 2003


Protein tolerance to random amino acid change
journal, June 2004

  • Guo, H. H.; Choe, J.; Loeb, L. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
  • DOI: 10.1073/pnas.0403255101

Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.
journal, March 1988

  • Lazar, E.; Watanabe, S.; Dalton, S.
  • Molecular and Cellular Biology, Vol. 8, Issue 3, p. 1247-1252
  • DOI: 10.1128/MCB.8.3.1247

Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli
journal, March 1998

  • Hill, Margaret A.; Preiss, Jack
  • Biochemical and Biophysical Research Communications, Vol. 244, Issue 2, p. 573-577
  • DOI: 10.1006/bbrc.1998.8301

Disentangling the perturbational effects of amino acid substitutions in the DNA-binding domain of p53
journal, February 1999

  • Wacey, A. I.; Cooper, D. N.; Liney, D.
  • Human Genetics, Vol. 104, Issue 1, p. 15-22
  • DOI: 10.1007/s004390050904

Mechanisms of Solvent Tolerance in Gram-Negative Bacteria
journal, October 2002


Advanced biofuel production in microbes
journal, January 2010

  • Peralta-Yahya, Pamela P.; Keasling, Jay D.
  • Biotechnology Journal, Vol. 5, Issue 2, p. 147-162
  • DOI: 10.1002/biot.200900220

Mechanisms of Solvent Tolerance in Gram-Negative Bacteria
journal, October 2002


Biofuel alternatives to ethanol: pumping the microbial well
journal, July 2008

  • Fortman, J. L.; Chhabra, Swapnil; Mukhopadhyay, Aindrila
  • Trends in Biotechnology, Vol. 26, Issue 7, p. 375-381
  • DOI: 10.1016/j.tibtech.2008.03.008

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels
journal, December 2008

  • Lee, Sung Kuk; Chou, Howard; Ham, Timothy S.
  • Current Opinion in Biotechnology, Vol. 19, Issue 6, p. 556-563
  • DOI: 10.1016/j.copbio.2008.10.014

Challenges in Engineering Microbes for Biofuels Production
journal, February 2007


A model for improving microbial biofuel production using a synthetic feedback loop
journal, February 2010

  • Dunlop, Mary J.; Keasling, Jay D.; Mukhopadhyay, Aindrila
  • Systems and Synthetic Biology, Vol. 4, Issue 2
  • DOI: 10.1007/s11693-010-9052-5

High-Density Renewable Fuels Based on the Selective Dimerization of Pinenes
journal, January 2010

  • Harvey, Benjamin G.; Wright, Michael E.; Quintana, Roxanne L.
  • Energy & Fuels, Vol. 24, Issue 1
  • DOI: 10.1021/ef900799c

Assembly and Channel Opening in a Bacterial Drug Efflux Machine
journal, April 2008


Molecular Properties of Bacterial Multidrug Transporters
journal, December 2000


Three Efflux Pumps Are Required To Provide Efficient Tolerance to Toluene in Pseudomonas putida DOT-T1E
journal, July 2001


Identification and Molecular Characterization of an Efflux Pump Involved in Pseudomonas putida S12 Solvent Tolerance
journal, January 1998

  • Kieboom, Jasper; Dennis, Jonathan J.; de Bont, Jan A. M.
  • Journal of Biological Chemistry, Vol. 273, Issue 1
  • DOI: 10.1074/jbc.273.1.85

The solvent efflux system of Pseudomonas putida S12 is not involved in antibiotic resistance
journal, November 2000

  • Isken, S.; De Bont, J. A. M.
  • Applied Microbiology and Biotechnology, Vol. 54, Issue 5
  • DOI: 10.1007/s002530000453

Mechanisms of RND multidrug efflux pumps
journal, May 2009

  • Nikaido, Hiroshi; Takatsuka, Yumiko
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1794, Issue 5, p. 769-781
  • DOI: 10.1016/j.bbapap.2008.10.004

Functional Genomic Study of Exogenous n-Butanol Stress in Escherichia coli
journal, January 2010

  • Rutherford, B. J.; Dahl, R. H.; Price, R. E.
  • Applied and Environmental Microbiology, Vol. 76, Issue 6
  • DOI: 10.1128/AEM.02323-09

Directed evolution of a bacterial efflux pump: Adaptation of the E. coli TolC exit duct to the Pseudomonas MexAB translocase
journal, September 2006


Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
journal, January 2008

  • Atsumi, Shota; Hanai, Taizo; Liao, James C.
  • Nature, Vol. 451, Issue 7174, p. 86-89
  • DOI: 10.1038/nature06450

Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol
journal, January 2008

  • Steen, Eric J.; Chan, Rossana; Prasad, Nilu
  • Microbial Cell Factories, Vol. 7, Issue 1, Article No. 36
  • DOI: 10.1186/1475-2859-7-36

Interplay between Efflux Pumps May Provide Either Additive or Multiplicative Effects on Drug Resistance
journal, June 2000


MicrobesOnline: an integrated portal for comparative and functional genomics
journal, November 2009

  • Dehal, P. S.; Joachimiak, M. P.; Price, M. N.
  • Nucleic Acids Research, Vol. 38, Issue suppl_1, p. D396-D400
  • DOI: 10.1093/nar/gkp919

Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen
journal, January 2001

  • Krogh, Anders; Larsson, Björn; von Heijne, Gunnar
  • Journal of Molecular Biology, Vol. 305, Issue 3
  • DOI: 10.1006/jmbi.2000.4315

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997

  • Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
  • Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
  • DOI: 10.1093/nar/25.17.3389

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000

  • Datsenko, K. A.; Wanner, B. L.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
  • DOI: 10.1073/pnas.120163297

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection
journal, February 2006

  • Baba, Tomoya; Ara, Takeshi; Hasegawa, Miki
  • Molecular Systems Biology, Vol. 2, Article No. 2006.0008
  • DOI: 10.1038/msb4100050

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
journal, February 2007

  • Li, Mamie Z.; Elledge, Stephen J.
  • Nature Methods, Vol. 4, Issue 3
  • DOI: 10.1038/nmeth1010