Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1

Fu, Yanfen; Beck, David A. C.; Lidstrom, Mary E.

doi:10.1186/s12866-016-0778-4

Title: Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1

Journal Article · Tue Jul 19 00:00:00 EDT 2016 · BMC Microbiology

DOI:https://doi.org/10.1186/s12866-016-0778-4· OSTI ID:1618591

Fu, Yanfen; Beck, David A. C.; Lidstrom, Mary E.

In this study, two variants of Methylobacterium extorquens AM1 demonstrated a trade-off between growth rate and biomass yield. In addition, growth rate and biomass yield were also affected by supplementation of growth medium with different amounts of cobalt. The metabolism changes relating to these growth phenomena as well as the trade-off were investigated in this study. ¹³C metabolic flux analysis was used to generate a detailed central carbon metabolic flux map with both absolute and normalized flux values. As a result, the major differences between the two variants occurred at the formate node as well as within C3-C4 inter-conversion pathways. Higher relative fluxes through formyltetrahydrofolate ligase, phosphoenolpyruvate carboxylase, and malic enzyme led to higher biomass yield, while higher relative fluxes through pyruvate kinase and pyruvate dehydrogenase led to higher growth rate. These results were then tested by phenotypic studies on three mutants (null pyk, null pck mutant and null dme mutant) in both variants, which agreed with the model prediction. In this study, ¹³C metabolic flux analysis for two strain variants of M. extorquens AM1 successfully identified metabolic pathways contributing to the trade-off between cell growth and biomass yield. Phenotypic analysis of mutants deficient in corresponding genes supported the conclusion that C3-C4 inter-conversion strategies were the major response to the trade-off.

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Research Organization:: Univ. of Washington, Seattle, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0006871

OSTI ID:: 1618591

Alternate ID(s):: OSTI ID: 1299217

Journal Information:: BMC Microbiology, Journal Name: BMC Microbiology Vol. 16 Journal Issue: 1; ISSN 1471-2180

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 10 works

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59 BASIC BIOLOGICAL SCIENCES
Methylobacterium extorquens AM1
Methylotrophy
physiological trade-off
metabolic flux analysis
cobalt
flux analysis
labeling experiments
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methanol
formate
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Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 [Supplemental Data] Beck, David; Lidstrom, Mary; Fu, Yanfen https://doi.org/10.6084/m9.figshare.c.3629378 The current record is supplemented by this collection	collection	July 2016
Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 [Supplemental Data] Fu, Yanfen; Beck, David; Lidstrom, Mary https://doi.org/10.6084/m9.figshare.c.3629378.v1 The current record is supplemented by this dataset	dataset	July 2016
Additional file 5: of Difference in C3âC4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 Fu, Yanfen; Beck, David; Lidstrom, Mary https://doi.org/10.6084/m9.figshare.c.3629378_d1 The current record is supplemented by this dataset	dataset	July 2016
Additional file 5: of Difference in C3âC4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 Fu, Yanfen; Beck, David; Lidstrom, Mary https://doi.org/10.6084/m9.figshare.c.3629378_d1.v1 The current record is supplemented by this dataset	dataset	July 2016
Additional file 4: of Difference in C3-C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 Beck, David; Lidstrom, Mary https://doi.org/10.6084/m9.figshare.c.3629378_d2.v1 The current record is supplemented by this dataset	dataset	July 2016
Additional file 1: Table S1. of Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 Fu, Yanfen; Beck, David; Lidstrom, Mary https://doi.org/10.6084/m9.figshare.c.3629378_d3 The current record is supplemented by this dataset	dataset	July 2016
Additional file 1: Table S1. of Difference in C3â C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 Fu, Yanfen; Beck, David; Lidstrom, Mary https://doi.org/10.6084/m9.figshare.c.3629378_d3.v1 The current record is supplemented by this dataset	dataset	July 2016
Additional file 2: of Difference in C3âC4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 Fu, Yanfen; Beck, David; Lidstrom, Mary https://doi.org/10.6084/M9.FIGSHARE.C.3629378_D4 The current record is supplemented by this dataset	dataset	July 2016
Additional file 2: of Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 Fu, Yanfen; Beck, David; Lidstrom, Mary https://doi.org/10.6084/m9.figshare.c.3629378_d4.v1 The current record is supplemented by this dataset	dataset	July 2016
Additional file 3: of Difference in C3â C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1 Fu, Yanfen; Beck, David; Lidstrom, Mary https://doi.org/10.6084/m9.figshare.c.3629378_d5.v1 The current record is supplemented by this dataset	dataset	July 2016