Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance

Hu, Bo; Yang, Yi -Ming; Beck, David A. C.; Wang, Qian -Wen; Chen, Wen -Jing; Yang, Jing; Lidstrom, Mary E.; Yang, Song

doi:10.1186/s13068-016-0497-y

Title: Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance

Journal Article · Mon Apr 11 00:00:00 EDT 2016 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-016-0497-y· OSTI ID:1253902

Hu, Bo ^[1]; Yang, Yi -Ming ^[2]; Beck, David A. C. ^[3]; Wang, Qian -Wen ^[2]; Chen, Wen -Jing ^[2]; Yang, Jing ^[2]; Lidstrom, Mary E. ^[3]; Yang, Song ^[4]

Univ. of Washington, Seattle, WA (United States); Intrexon Corp., South San Francisco, CA (United States)
Qingdao Agricultural Univ., Qingdao (China)
Univ. of Washington, Seattle, WA (United States)
Qingdao Agricultural Univ., Qingdao (China); Tianjin Univ., Tianjin (China)

In this study, the toxicity of alcohols is one of the major roadblocks of biological fermentation for biofuels production. Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, has been engineered to generate 1-butanol from cheap carbon feedstocks through a synthetic metabolic pathway. However, M. extorquens AM1 is vulnerable to solvent stress, which impedes further development for 1-butanol production. Only a few studies have reported the general stress response of M. extorquens AM1 to solvent stress. Therefore, it is highly desirable to obtain a strain with ameliorated 1-butanol tolerance and elucidate the molecular mechanism of 1-butnaol tolerance in M. extorquens AM1 for future strain improvement. In this work, adaptive laboratory evolution was used as a tool to isolate mutants with 1-butanol tolerance up to 0.5 %. The evolved strains, BHBT3 and BHBT5, demonstrated increased growth rates and higher survival rates with the existence of 1-butanol. Whole genome sequencing revealed a SNP mutation at kefB in BHBT5, which was confirmed to be responsible for increasing 1-butanol tolerance through an allelic exchange experiment. Global metabolomic analysis further discovered that the pools of multiple key metabolites, including fatty acids, amino acids, and disaccharides, were increased in BHBT5 in response to 1-butanol stress. Additionally, the carotenoid synthesis pathway was significantly down-regulated in BHBT5. In conclusion, we successfully screened mutants resistant to 1-butanol and provided insights into the molecular mechanism of 1-butanol tolerance in M. extorquens AM1. This research will be useful for uncovering the mechanism of cellular response of M. extorquens AM1 to solvent stress, and will provide the genetic blueprint for the rational design of a strain of M. extorquens AM1 with increased 1-butanol tolerance in the future.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Univ. of Washington, Seattle, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0006871

OSTI ID:: 1253902

Journal Information:: Biotechnology for Biofuels, Vol. 9, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 29 works

Citation information provided by
Web of Science

References (52)

Microbial laboratory evolution in the era of genome‐scale science Conrad, Tom M.; Lewis, Nathan E.; Palsson, Bernhard Ø Molecular Systems Biology, Vol. 7, Issue 1 https://doi.org/10.1038/msb.2011.42	journal	January 2011
MetaboAnalyst 3.0—making metabolomics more meaningful Xia, Jianguo; Sinelnikov, Igor V.; Han, Beomsoo Nucleic Acids Research, Vol. 43, Issue W1 https://doi.org/10.1093/nar/gkv380	journal	April 2015
Engineering alternative butanol production platforms in heterologous bacteria Nielsen, David R.; Leonard, Effendi; Yoon, Sang-Hwal Metabolic Engineering, Vol. 11, Issue 4-5, p. 262-273 https://doi.org/10.1016/j.ymben.2009.05.003	journal	July 2009
Gene expression profiles and intracellular contents of stress protectants in Saccharomyces cerevisiae under ethanol and sorbitol stresses Kaino, Tomohiro; Takagi, Hiroshi Applied Microbiology and Biotechnology, Vol. 79, Issue 2 https://doi.org/10.1007/s00253-008-1431-4	journal	March 2008
Bioconversion of natural gas to liquid fuel: Opportunities and challenges Fei, Qiang; Guarnieri, Michael T.; Tao, Ling Biotechnology Advances, Vol. 32, Issue 3 https://doi.org/10.1016/j.biotechadv.2014.03.011	journal	May 2014
Solvent tolerance in Gram-negative bacteria Segura, Ana; Molina, Lázaro; Fillet, Sandy Current Opinion in Biotechnology, Vol. 23, Issue 3 https://doi.org/10.1016/j.copbio.2011.11.015	journal	June 2012
Adaptive laboratory evolution—harnessing the power of biology for metabolic engineering Portnoy, Vasiliy A.; Bezdan, Daniela; Zengler, Karsten Current Opinion in Biotechnology, Vol. 22, Issue 4 https://doi.org/10.1016/j.copbio.2011.03.007	journal	August 2011
Laboratory divergence of Methylobacterium extorquens AM1 through unintended domestication and past selection for antibiotic resistance Carroll, Sean; Xue, Katherine S.; Marx, Christopher J. BMC Microbiology, Vol. 14, Issue 1 https://doi.org/10.1186/1471-2180-14-2	journal	January 2014
Comprehensive phenotypic analysis for identification of genes affecting growth under ethanol stress in Saccharomyces cerevisiae Yoshikawa, Katsunori; Tanaka, Tadamasa; Furusawa, Chikara FEMS Yeast Research, Vol. 9, Issue 1 https://doi.org/10.1111/j.1567-1364.2008.00456.x	journal	February 2009
Understanding the Structural Requirements for Activators of the Kef Bacterial Potassium Efflux System Healy, Jessica; Ekkerman, Silvia; Pliotas, Christos Biochemistry, Vol. 53, Issue 12 https://doi.org/10.1021/bi5001118	journal	March 2014
Mechanisms of ethanol tolerance in Saccharomyces cerevisiae Ma, Menggen; Liu, Z. Lewis Applied Microbiology and Biotechnology, Vol. 87, Issue 3 https://doi.org/10.1007/s00253-010-2594-3	journal	May 2010
MZmine 2: Modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data Pluskal, Tomáš; Castillo, Sandra; Villar-Briones, Alejandro BMC Bioinformatics, Vol. 11, Issue 1 https://doi.org/10.1186/1471-2105-11-395	journal	July 2010
Thioesterases for ethylmalonyl–CoA pathway derived dicarboxylic acid production in Methylobacterium extorquens AM1 Sonntag, Frank; Buchhaupt, Markus; Schrader, Jens Applied Microbiology and Biotechnology, Vol. 98, Issue 10 https://doi.org/10.1007/s00253-013-5456-y	journal	January 2014
Metabolic engineering of Methylobacterium extorquens AM1 for 1-butanol production Hu, Bo; Lidstrom, Mary E. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0156-0	journal	October 2014
Proline inhibits aggregation during protein refolding Samuel, Dharmaraj; Ganesh, Gopal; Yang, Pey-Wen Protein Science, Vol. 9, Issue 2 https://doi.org/10.1110/ps.9.2.344	journal	January 2000
Development of a broad-host-range sacB-based vector for unmarked allelic exchange Marx, Christopher J. BMC Research Notes, Vol. 1, Article No. 1 https://doi.org/10.1186/1756-0500-1-1	journal	January 2008
Genetic Characterization of the Carotenoid Biosynthetic Pathway in Methylobacterium extorquens AM1 and Isolation of a Colorless Mutant Van Dien, S. J.; Marx, C. J.; O'Brien, B. N. Applied and Environmental Microbiology, Vol. 69, Issue 12 https://doi.org/10.1128/AEM.69.12.7563-7566.2003	journal	December 2003
Fast and accurate long-read alignment with Burrows–Wheeler transform Li, Heng; Durbin, Richard Bioinformatics, Vol. 26, Issue 5 https://doi.org/10.1093/bioinformatics/btp698	journal	January 2010
Global discovery of adaptive mutations Goodarzi, Hani; Hottes, Alison K.; Tavazoie, Saeed Nature Methods, Vol. 6, Issue 8 https://doi.org/10.1038/nmeth.1352	journal	July 2009
The Expanding World of Methylotrophic Metabolism Chistoserdova, Ludmila; Kalyuzhnaya, Marina G.; Lidstrom, Mary E. Annual Review of Microbiology, Vol. 63, Issue 1 https://doi.org/10.1146/annurev.micro.091208.073600	journal	October 2009
Characterizing the in vivo role of trehalose in Saccharomyces cerevisiae using the AGT1 transporter Gibney, Patrick A.; Schieler, Ariel; Chen, Jonathan C. Proceedings of the National Academy of Sciences, Vol. 112, Issue 19 https://doi.org/10.1073/pnas.1506289112	journal	April 2015
Liquid chromatography–tandem quadrupole mass spectrometry and comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry measurement of targeted metabolites of Methylobacterium extorquens AM1 grown on two different carbon sources Yang, Song; Sadilek, Martin; Synovec, Robert E. Journal of Chromatography A, Vol. 1216, Issue 15 https://doi.org/10.1016/j.chroma.2009.02.030	journal	April 2009
Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production Yomano, L. P.; York, S. W.; Ingram, L. O. Journal of Industrial Microbiology and Biotechnology, Vol. 20, Issue 2 https://doi.org/10.1038/sj.jim.2900496	journal	February 1998
Engineering alcohol tolerance in yeast Lam, Felix H.; Ghaderi, Adel; Fink, Gerald R. Science, Vol. 346, Issue 6205 https://doi.org/10.1126/science.1257859	journal	October 2014
Effect of L-Proline on Sake Brewing and Ethanol Stress in Saccharomyces cerevisiae Takagi, H.; Takaoka, M.; Kawaguchi, A. Applied and Environmental Microbiology, Vol. 71, Issue 12 https://doi.org/10.1128/AEM.71.12.8656-8662.2005	journal	December 2005
Streamlined pentafluorophenylpropyl column liquid chromatography–tandem quadrupole mass spectrometry and global 13C-labeled internal standards improve performance for quantitative metabolomics in bacteria Yang, Song; Sadilek, Martin; Lidstrom, Mary E. Journal of Chromatography A, Vol. 1217, Issue 47 https://doi.org/10.1016/j.chroma.2010.09.055	journal	November 2010
Visualizing evolution in real time to determine the molecular mechanisms of n-butanol tolerance in Escherichia coli Reyes, Luis H.; Almario, Maria P.; Winkler, James Metabolic Engineering, Vol. 14, Issue 5 https://doi.org/10.1016/j.ymben.2012.05.002	journal	September 2012
Methanol-based industrial biotechnology: current status and future perspectives of methylotrophic bacteria Schrader, Jens; Schilling, Martin; Holtmann, Dirk Trends in Biotechnology, Vol. 27, Issue 2, p. 107-115 https://doi.org/10.1016/j.tibtech.2008.10.009	journal	February 2009
MS/MS networking guided analysis of molecule and gene cluster families Nguyen, D. D.; Wu, C. -H.; Moree, W. J. Proceedings of the National Academy of Sciences, Vol. 110, Issue 28 https://doi.org/10.1073/pnas.1303471110	journal	June 2013
Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli Atsumi, Shota; Wu, Tung‐Yun; Machado, Iara M. P. Molecular Systems Biology, Vol. 6, Issue 1 https://doi.org/10.1038/msb.2010.98	journal	January 2010
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
Adaptive laboratory evolution – principles and applications for biotechnology Dragosits, Martin; Mattanovich, Diethard Microbial Cell Factories, Vol. 12, Issue 1 https://doi.org/10.1186/1475-2859-12-64	journal	January 2013
Identification of Mutations in Laboratory-Evolved Microbes from Next-Generation Sequencing Data Using breseq Deatherage, Daniel E.; Barrick, Jeffrey E. Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-0554-6_12	book	January 2014
Identification of target genes conferring ethanol stress tolerance to Saccharomyces cerevisiae based on DNA microarray data analysis Hirasawa, Takashi; Yoshikawa, Katsunori; Nakakura, Yuki Journal of Biotechnology, Vol. 131, Issue 1 https://doi.org/10.1016/j.jbiotec.2007.05.010	journal	August 2007
Maintenance of pH by a butanol-tolerant mutant of Clostridium beijerinckii Wang, F. Microbiology, Vol. 151, Issue 2 https://doi.org/10.1099/mic.0.27587-0	journal	February 2005
An integrated network approach identifies the isobutanol response network of Escherichia coli Brynildsen, Mark P.; Liao, James C. Molecular Systems Biology, Vol. 5, Issue 1 https://doi.org/10.1038/msb.2009.34	journal	January 2009
Parallel and Divergent Evolutionary Solutions for the Optimization of an Engineered Central Metabolism in Methylobacterium extorquens AM1 Carroll, Sean; Chubiz, Lon; Agashe, Deepa Microorganisms, Vol. 3, Issue 2 https://doi.org/10.3390/microorganisms3020152	journal	April 2015
Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans Figurski, D. H.; Helinski, D. R. Proceedings of the National Academy of Sciences, Vol. 76, Issue 4 https://doi.org/10.1073/pnas.76.4.1648	journal	April 1979
Genomic Library Screens for Genes Involved in n-Butanol Tolerance in Escherichia coli Reyes, Luis H.; Almario, Maria P.; Kao, Katy C. PLoS ONE, Vol. 6, Issue 3 https://doi.org/10.1371/journal.pone.0017678	journal	March 2011
Carotenoids as modulators of lipid membrane physical properties Gruszecki, Wiesław I.; Strzałka, Kazimierz Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Vol. 1740, Issue 2 https://doi.org/10.1016/j.bbadis.2004.11.015	journal	May 2005
Implementation of Microarrays for Methylobacterium extorquens AM1 Okubo, Yoko; Skovran, Elizabeth; Guo, Xiaofeng OMICS: A Journal of Integrative Biology, Vol. 11, Issue 4 https://doi.org/10.1089/omi.2007.0027	journal	December 2007
The Sequence Alignment/Map format and SAMtools Li, H.; Handsaker, B.; Wysoker, A. Bioinformatics, Vol. 25, Issue 16 https://doi.org/10.1093/bioinformatics/btp352	journal	June 2009
Genetic Tools for the Industrially Promising Methanotroph Methylomicrobium buryatense Puri, Aaron W.; Owen, Sarah; Chu, Frances Applied and Environmental Microbiology, Vol. 81, Issue 5 https://doi.org/10.1128/AEM.03795-14	journal	December 2014
Methylobacterium extorquens: methylotrophy and biotechnological applications Ochsner, Andrea M.; Sonntag, Frank; Buchhaupt, Markus Applied Microbiology and Biotechnology, Vol. 99, Issue 2 https://doi.org/10.1007/s00253-014-6240-3	journal	November 2014
Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells Zhao, Ruifeng; Zuo, Qisheng; Yuan, Xia Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-23242-5	journal	May 2021
A multi-omic characterization of temperature stress in a halotolerant Scenedesmus strain for algal biotechnology Calhoun, Sara; Bell, Tisza Ann Szeremy; Dahlin, Lukas R. Communications Biology, Vol. 4, Issue 1 https://doi.org/10.1038/s42003-021-01859-y	journal	March 2021
MS/MS networking guided analysis of molecule and gene cluster families M., Gutierrez,; S., Moore, B.; C., Dorrestein, P. The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/bd99-qk88	text	January 2013
A General Framework for Designing and Validating Oligomer-Based DNA Microarrays and Its Application to Clostridium acetobutylicum Paredes, C. J.; Senger, R. S.; Spath, I. S. Applied and Environmental Microbiology, Vol. 73, Issue 14 https://doi.org/10.1128/aem.00144-07	journal	May 2007
Functional Genomic Study of Exogenous n-Butanol Stress in Escherichia coli Rutherford, B. J.; Dahl, R. H.; Price, R. E. Applied and Environmental Microbiology, Vol. 76, Issue 6 https://doi.org/10.1128/aem.02323-09	journal	January 2010
PhyR Is Involved in the General Stress Response of Methylobacterium extorquens AM1 Gourion, B.; Francez-Charlot, A.; Vorholt, J. A. Journal of Bacteriology, Vol. 190, Issue 3 https://doi.org/10.1128/jb.01483-07	journal	November 2007
Molecular cloning of a malyl coenzyme A lyase gene from Pseudomonas sp. strain AM1, a facultative methylotroph. Fulton, G. L.; Nunn, D. N.; Lidstrom, M. E. Journal of Bacteriology, Vol. 160, Issue 2 https://doi.org/10.1128/jb.160.2.718-723.1984	journal	January 1984
Global Molecular Analyses of Methane Metabolism in Methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part II. Metabolomics and 13C-Labeling Study Yang, Song; Matsen, Janet B.; Konopka, Michael Frontiers in Microbiology, Vol. 4 https://doi.org/10.3389/fmicb.2013.00070	journal	January 2013

Cited By (6)

Breeding of Methanol-Tolerant Methylobacterium extorquens AM1 by Atmospheric and Room Temperature Plasma Mutagenesis Combined With Adaptive Laboratory Evolution Cui, Lan-Yu; Wang, Shan-Shan; Guan, Chang-Ge Biotechnology Journal https://doi.org/10.1002/biot.201700679	journal	May 2018
Guidance for engineering of synthetic methylotrophy based on methanol metabolism in methylotrophy Zhang, Wenming; Zhang, Ting; Wu, Sihua RSC Advances, Vol. 7, Issue 7 https://doi.org/10.1039/c6ra27038g	journal	January 2017
Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route Yang, Yi-Ming; Chen, Wen-Jing; Yang, Jing Microbial Cell Factories, Vol. 16, Issue 1 https://doi.org/10.1186/s12934-017-0798-2	journal	October 2017
Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor Yang, Jing; Zhang, Chang-Tai; Yuan, Xiao-Jie Microbial Cell Factories, Vol. 17, Issue 1 https://doi.org/10.1186/s12934-018-1042-4	journal	December 2018
Adaptive Laboratory Evolution of Antibiotic Resistance Using Different Selection Regimes Lead to Similar Phenotypes and Genotypes Jahn, Leonie J.; Munck, Christian; Ellabaan, Mostafa M. H. Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.00816	journal	May 2017
Metabolomics Investigation of an Association of Induced Features and Corresponding Fungus during the Co-culture of Trametes versicolor and Ganoderma applanatum Xu, Xiao-Yan; Shen, Xiao-Ting; Yuan, Xiao-Jie Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.02647	journal	January 2018

Linked Research (16)

Figures / Tables (11)

Similar Records

Metabolomics Revealed an Association of Metabolite Changes and Defective Growth in Methylobacterium extorquens AM1 Overexpressing ecm during Growth on Methanol

Journal Article · Tue Apr 26 00:00:00 EDT 2016 · PLoS ONE · OSTI ID:1253902

Cui, Jinyu; Good, Nathan M.; Hu, Bo; +5 more

Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1

Journal Article · Tue Jul 19 00:00:00 EDT 2016 · BMC Microbiology · OSTI ID:1253902

Fu, Yanfen; Beck, David A. C.; Lidstrom, Mary E.

Engineering Terpene Production Pathways in Methylobacterium extorquens AM1

Journal Article · Thu Feb 29 00:00:00 EST 2024 · Microorganisms · OSTI ID:1253902

Hurt, Allison; Bibik, Jacob D.; Martinez-Gomez, Norma Cecilia; +1 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
Methylobacterium extorquens AM1
1-butanol tolerance
adaptive evolution
whole genome sequencing
global metabolome analysis
carotenoid

Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance [Supplemental Data] Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028 The current record is supplemented by this dataset	dataset	April 2016
Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance [Supplemental Data] Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028.v1 The current record is supplemented by this dataset	dataset	April 2016
MOESM7 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028_d1 The current record is supplemented by this dataset	dataset	January 2016
MOESM7 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028_d1.v1 The current record is supplemented by this dataset	dataset	April 2016
MOESM4 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/M9.FIGSHARE.C.3613028_D2 The current record is supplemented by this image	image	January 2016
MOESM4 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028_d2.v1 The current record is supplemented by this figure	figure	April 2016
MOESM3 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/M9.FIGSHARE.C.3613028_D3 The current record is supplemented by this image	image	January 2016
MOESM3 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028_d3.v1 The current record is supplemented by this dataset	dataset	April 2016
MOESM5 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/M9.FIGSHARE.C.3613028_D4 The current record is supplemented by this image	image	January 2016
MOESM5 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028_d4.v1 The current record is supplemented by this dataset	dataset	April 2016
MOESM1 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/M9.FIGSHARE.C.3613028_D5 The current record is supplemented by this dataset	dataset	January 2016
MOESM1 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028_d5.v1 The current record is supplemented by this dataset	dataset	April 2016
MOESM2 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028_d6 The current record is supplemented by this image	image	January 2016
MOESM2 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028_d6.v1 The current record is supplemented by this figure	figure	April 2016
MOESM6 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/M9.FIGSHARE.C.3613028_D7 The current record is supplemented by this image	image	January 2016
MOESM6 of Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance Hu, Bo; Yang, Yi-Ming; Beck, David https://doi.org/10.6084/m9.figshare.c.3613028_d7.v1 The current record is supplemented by this figure	figure	April 2016