Systems and methods for the secretion of recombinant proteins in gram negative bacteria
Patent
·
OSTI ID:1289850
Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.
- Research Organization:
- WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FC02-07ER64494
- Assignee:
- WISCONSIN ALUMNI RESEARCH FOUNDATION (Madison, WI)
- Patent Number(s):
- 9,410,157
- Application Number:
- 13/192,058
- OSTI ID:
- 1289850
- Country of Publication:
- United States
- Language:
- English
Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli
|
journal | December 2005 |
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